CCR5 Antikörper (N-Term)

Produktnummer ABIN3044318

Produktdetails anti-CCR5 Antikörper

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Bindungsspezifität: AA 19-34, N-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCR5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband®

Sequenz: PCQKINVKQI AARLLP

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-C-C chemokine receptor type 5 CCR5 Antibody (ABIN3044318). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CCR5, different from the related rat sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Samson M, Labbe O, Mollereau C, Vassart G, Parmentier M. Molecular cloning and functional expression of a new human CC-chemokine receptor gene. Biochemistry. 1996 Mar 19, 35(11):3362-7. 2. Rottman JB, Ganley KP, Williams K, Wu L, Mackay CR, Ringler DJ. Cellular localization of the chemokine receptor CCR5. Correlation to cellular targets of HIV-1 infection. Am J Pathol. 1997 Nov, 151(5):1341-51. 3. Mummidi S, Ahuja SS, McDaniel BL, Ahuja SK. The human CC chemokine receptor 5 (CCR5) gene. Multiple transcripts with 5'-end heterogeneity, dual promoter usage, and evidence for polymorphisms within the regulatory regions and noncoding exons. J Biol Chem. 1997 Dec 5, 272(49):30662-71.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-CCR5 Antikörper (ABIN3044318)

Li, Xu, Zhu, Xu, Qin, Liu, Tu, Peng, Qiu, Yu, Zhu, Fan, Zheng, Li, Liang: "Effects of neferine on CCL5 and CCR5 expression in SCG of type 2 diabetic rats." in: Brain research bulletin, Vol. 90, pp. 79-87, (2012) (PubMed).

Details zu CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Andere Bezeichnung: CCR5 (CCR5 Produkte)

Hintergrund:

Synonyms: C-C chemokine receptor type 5,C-C CKR-5,CC-CKR-5,CCR-5,CCR5,CHEMR13,HIV-1 fusion coreceptor,CD195,CCR5,CMKBR5,

Tissue Specificity: Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1a and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung. .

Background: Human CC chemokine receptor 5 (CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1.

Sequence Similarities: Belongs to the G-protein coupled receptor 1 family.

Molekulargewicht: 40 kDa

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size