CAPNL1 Antikörper (Middle Region)

Produktnummer ABIN3044292

Zitiert in 3+ Publikationen. Der Kaninchen Polyklonal anti-CAPNL1 Antikörper (ABIN3044292) detektiert spezifisch CAPNL1 in WB, IHC, ICC, IF und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für CAPNL1 Antikörper (Middle Region) (ABIN3044292)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAPNL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CAPNL1 Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: Anti-Calpain 1/CAPN1 Antibody

Sequenz: EWNNVDPYER DQLRV

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: different from the mouse sequence by two amino acids.

Produktmerkmale: Anti-Calpain 1/CAPN1 Antibody. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1, different from the mouse sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Rat, Human, Mouse Immunocytochemistry , 0.5-1 μg/mL, Human, - Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-CAPNL1 Antikörper (ABIN3044292)

Ye, Wang, Zhang, Song, Yang, Li, Li, Su, Yang, Ma: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).

Chen, Tang, Gao, Xu, Jiang, Cao, Fu, Sun, Liu, Shi: "Fibrosis, adipogenesis, and muscle atrophy in congenital muscular torticollis." in: Medicine, Vol. 93, Issue 23, pp. e138, (2014) (PubMed).

Mo, Chen, Li, Zheng, Ke, Deng, Li, Jiang, Wu, Wang, Wang, Yang, Cao: "Suppression of NHE1 by small interfering RNA inhibits HIF-1?-induced angiogenesis in vitro via modulation of calpain activity." in: Microvascular research, Vol. 81, Issue 2, pp. 160-8, (2011) (PubMed).

Details zu CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Andere Bezeichnung: CAPN1

Hintergrund:

Background: Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1 (CAPN1) and L2. By quantitative RT-PCR, Ueyama et al. (1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis (ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al. (1989, 1990) assign CANPL1 to chromosome 11.

Gene Full Name: calpain 1

Sequence Similarities: Belongs to the peptidase C2 family.

Molekulargewicht: 82 kDa

Gen-ID: 823

UniProt: P07384

Pathways: Apoptose