RELB Antikörper (C-Term)

Produktnummer ABIN3044186

Der Kaninchen Polyklonal Anti-RELB-Antikörper wurde für WB validiert. Er ist geeignet, RELB in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für RELB Antikörper (C-Term) (ABIN3044186)

Target: RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RELB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RELB Antikörper

Bindungsspezifität: AA 393-413, C-Term

Verwendungszweck: Anti-Rel B/RELB Antibody Picoband®

Sequenz: LPFTYLPRDH DSYGVDKKRK R

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Rel B/RELB Antibody (ABIN3044186). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rel B, identical to the related mouse sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Clark, G. J., Gunningham, S., Troy, A., Vuckovic, S., Hart, D. N.Expression of the RelB transcription factor correlates with the activation of human dendritic cells.Immunology 98: 189-196, 1999. 2. Ruben, S. M., Klement, J. F., Coleman, T. A., Maher, M., Chen, C.-H., Rosen, C. A.I-Rel: a novel rel-related protein that inhibits NF-kappa-B transcriptional activity.Genes Dev. 6: 745-760, 1992. 3. Vaira, S., Johnson, T., Hirbe, A. C., Alhawagri, M., Anwisye, I., Sammut, B., O'Neal, J., Zou, W., Weilbaecher, K. N., Faccio, R., Novack, D. V. RelB is the NF-kappa-B subunit downstream of NIK responsible for osteoclast differentiation.Proc. Nat. Acad. Sci. 105: 3897-3902, 2008.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu RELB

Target: RELB (V-Rel Reticuloendotheliosis Viral Oncogene Homolog B (RELB))

Andere Bezeichnung: RELB

Hintergrund:

Synonyms: Transcription factor RelB,I-Rel,RELB,

Background: RELB (v-rel reticuloendotheliosis viral oncogene homolog B) is also known as IREL. The International Radiation Hybrid Mapping Consortium assigned the RELB gene to chromosome 19. By RT-PCR and immunocytochemical analyses, Clark et al. (1999) showed that RELB expression correlated with dendritic cell activation. NF-kappa-B-inducing kinase is required for osteoclastogenesis in response to pathologic stimuli. Vaira et al. (2008) found that overexpression of Relb, but not Rela, rescued differentiation of mouse Nik -/- osteoclast precursors, indicating that blockade of the alternative NF-kappa-B pathway, rather than the classical NF-kappa-B pathway, is responsible for the osteoclastogenic defect in the absence of Nik. Using Relb -/- mice, they showed that Relb itself was required for Rankl-induced osteoclastogenesis in vitro and for TNF-induced bone resorption in vivo. Both Relb -/- and Nik -/- mice were resistant to tumor-mediated osteolysis. Vaira et al. (2008) concluded that the alternative NF-kappa-B pathway, via RELB, plays an essential and unique role in RANKL signaling toward osteoclast development.

Sequence Similarities: Contains 1 RHD (Rel-like) domain.

Molekulargewicht: 23 kDa

UniProt: Q01201

Pathways: NF-kappaB Signalweg, RTK Signalweg