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MCM7 Antikörper (C-Term)

Dieses Anti-MCM7-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von MCM7 in WB, IHC, IF, FACS und ICC. Geeignet für Human.
Produktnummer ABIN3044178

Kurzübersicht für MCM7 Antikörper (C-Term) (ABIN3044178)

Target

Alle MCM7 Antikörper anzeigen
MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

Reaktivität

  • 155
  • 31
  • 27
  • 11
  • 10
  • 10
  • 9
  • 9
  • 4
  • 2
  • 1
Human

Wirt

  • 101
  • 52
  • 2
Kaninchen

Klonalität

  • 82
  • 73
Polyklonal

Konjugat

  • 85
  • 8
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MCM7 Antikörper ist unkonjugiert

Applikation

  • 117
  • 71
  • 37
  • 35
  • 27
  • 21
  • 18
  • 16
  • 14
  • 13
  • 13
  • 5
  • 5
  • 4
  • 4
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 62
    • 15
    • 9
    • 9
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 699-719, C-Term

    Verwendungszweck

    Anti-MCM7 Antibody Picoband®

    Sequenz

    DEYEELNVWQ VNASRTRITF V

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-MCM7 Antibody (ABIN3044178). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human MCM7.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Groth, A., Corpet, A., Cook, A. J. L., Roche, D., Bartek, J., Lukas, J., Almouzni, G. Regulation of replication fork progression through histone supply and demand. Science 318: 1928-1931, 2007. 2. Nakatsuru, S., Sudo, K., Nakamura, Y. Isolation and mapping of a human gene (MCM2) encoding a product homologous to yeast proteins involved in DNA replication.Cytogenet. Cell Genet. 68: 226-230, 1995. 3. Petrocca, F., Visone, R., Onelli, M. R., Shah, M. H., Nicoloso, M. S., de Martino, I., Iliopoulos, D., Pilozzi, E., Liu, C.-G., Negrini, M., Cavazzini, L., Volinia, S., Alder, H., Ruco, L. P., Baldassarre, G., Croce, C. M., Vecchione, A. E2F1-regulated microRNAs impair TGF-beta-dependent cell-cycle arrest and apoptosis in gastric cancer. Cancer Cell 13: 272-286, 2008.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))

    Andere Bezeichnung

    MCM7

    Hintergrund

    Synonyms: DNA replication licensing factor MCM7,3.6.4.12,CDC47 homolog,P1.1-MCM3,MCM7,CDC47, MCM2,

    Background: MCM7 (Minichromosome Maintenance, s. Cerevisiae, homolog of, 7), also called CDC47, FORMERLY, is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The MCM7 gene is mapped on 7q22.1. MCM7 plays a pivotal role in the G1/S phase transition, orchestrating the correct assembly of replication forks on chromosomal DNA and ensuring that all the genome is replicated once and not more than once at each cell cycle. The MCM7 gene contains 15 exons. The miRNAs MIR106B, MIR93, and MIR25 are clustered in a 5-prime to 3-prime orientation within intron 13. Petrocca et al. (2008) found that MCM7 and the precursors of microRNAs (miRNAs) MIR106B, MIR93, and MIR25, all of which arise from intron 13 of the MCM7 gene, were overexpressed with almost perfect correlation in 5 of 10 human gastric tumors.

    Sequence Similarities: Belongs to the MCM family.

    Molekulargewicht

    80 kDa

    UniProt

    P33993

    Pathways

    DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
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