Aurora A Antikörper (Middle Region)

Produktnummer ABIN3044171

Der Kaninchen Polyklonal Anti-Aurora A-Antikörper wurde für WB validiert. Er ist geeignet, Aurora A in Proben von Maus und Ratte zu detektieren.

Kurzübersicht für Aurora A Antikörper (Middle Region) (ABIN3044171)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aurora A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Aurora A Antikörper

Bindungsspezifität: AA 109-125, Middle Region

Verwendungszweck: Anti-Aurora A/AURKA Antibody Picoband®

Sequenz: QKTEDTKKRQ WTLEDFD

Kreuzreaktivität (Details): No cross-reactivity with otherproteins

Produktmerkmale: Anti-Aurora A/AURKA Antibody (ABIN3044171). Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Aurora A, different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat
1. Bischoff, J. R., Plowman, G. D. The Aurora/lpl 1p kinase family: regulators of chromosome segregation and cytokinesis. Trends Cell Biol. 9: 454-459, 1999. 2. Hutterer, A., Berdnik, D., Wirtz-Peitz, F., Zigman, M., Schleiffer, A., Knoblich, J. A. Mitotic activation of the kinase Aurora-A requires its binding partner Bora. Dev. Cell 11: 147-157, 2006. 3. Seki, A., Coppinger, J. A., Jang, C.-Y., Yates, J. R., III, Fang, G. Bora and the kinase Aurora A cooperatively activate the kinase Plk1 and control mitotic entry. Science 320: 1655-1658, 2008.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Andere Bezeichnung: AURKA

Hintergrund:

Synonyms: Aurora kinase A,2.7.11.1,Aurora 2,Aurora family kinase 1,Aurora/IPL1-related kinase 1,ARK-1,Aurora-related kinase 1,Ipl1- and aurora-related kinase 1,Serine/threonine-protein kinase 6,Serine/threonine-protein kinase Ayk1,Serine/threonine-protein kinase aurora-A,Aurka,Aik, Airk, Ark1, Aura, Ayk1, Btak, Iak1, Stk15, Stk6,

Tissue Specificity: Detected in embryonic neurons in dorsal root ganglia and brain cortex (at protein level). Highly expressed in testis, in about one third of the seminiferous tubules. Expression is restricted to specific spermatocytes nearing completion of prophase, with levels falling off on transition to elongated spermatids. Highly expressed in the ovary, expression in the oocyte starts around the transition to large growing follicle. Abundant expression is seen in the proliferating granulosa and thecal cells of the growing follicle, and in the young corpus luteum. Very weakly expressed in spleen and intestine. .

Background: AURKA (aurora kinase A), also called ARK1, AurA, AIK , AURORA2 ,BTAK, PPP1R47, STK7, STK15,STK6, is a mitotic centrosomal protein kinase. The main role of AURKA in tumor development is in controlling chromosome segregation during mitosis. Aurora A is a member of a family of mitotic serine/threonine kinases. Cell cycle and Northern blot analyses showed that peak expression of AURKA occurs during the G2/M phase and then decreases. By fluorescence in situ hybridization, AURKA gene is represented by 2 signals in chromosome bands 20q13.2-q13.3 and 1q41-q42. The AURKA gene is overexpressed in many human cancers. Ectopic overexpression of Aurora kinase A in mammalian cells induces centrosome amplification, chromosome instability, and oncogenic transformation, a phenotype characteristic of loss-of-function mutations of p53. Depletion of Ajuba prevented activation of AURKA at centrosomes in late G2 phase and inhibited mitotic entry. Activation of AURKA was independently sufficient to induce rapid ciliary resorption, and AURKA acted in this process through phosphorylation of HDAC6, leading to HDAC6-dependent tubulin deacetylation and destabilization of the ciliary axoneme. Small molecule inhibitors of AURKA and HDAC6 reduced regulated disassembly of cilia.

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily.

Molekulargewicht: 46 kDa

UniProt: P97477

Pathways: Zellzyklus, Asymmetric Protein Localization