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GLI3 Antikörper (N-Term)

GLI3 Reaktivität: Human, Maus, Ratte WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3044156
  • Target Alle GLI3 Antikörper anzeigen
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Bindungsspezifität
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 40-57, N-Term
    Reaktivität
    • 34
    • 13
    • 5
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 28
    • 5
    • 2
    Kaninchen
    Klonalität
    • 23
    • 12
    Polyklonal
    Konjugat
    • 21
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser GLI3 Antikörper ist unkonjugiert
    Applikation
    • 16
    • 11
    • 8
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Verwendungszweck
    Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®
    Sequenz
    SNEDESPGQT YHRERRNA
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-Transcriptional activator GLI3 Gli3 Antibody (ABIN3044156). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.
    Isotyp
    IgG
    Top Product
    Discover our top product GLI3 Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
    1. Barna, M., Pandolfi, P. P., Niswander, L. Gli3 and Plzf cooperate in proximal limb patterning at early stages of limb development. (Letter) Nature 436: 277-281, 2005. 2. Bose, J., Grotewold, L., Ruther, U. Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum. Molec. Genet. 11: 1129-1135, 2002. 3. Craig, D. W., Itty, A., Panganiban, C., Szelinger, S., Kruer, M. C., Sekar, A., Reiman, D., Narayanan, V., Stephan, D. A., Kerrigan, J. F.Identification of somatic chromosomal abnormalities in hypothalamic hamartoma tissue at the GLI3 locus. Am. J. Hum. Genet. 82: 366-374, 2008.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    Konservierungsmittel
    Thimerosal (Merthiolate), Sodium azide
    Vorsichtsmaßnahmen
    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    12 months
  • Kumari, Chaurasia, Kumar, Dash: "Anti-apoptotic role of sonic hedgehog on blood platelets." in: Thrombosis research, Vol. 134, Issue 6, pp. 1311-5, (2014) (PubMed).

  • Target
    GLI3 (GLI Family Zinc Finger 3 (GLI3))
    Andere Bezeichnung
    GLI3 (GLI3 Produkte)
    Hintergrund

    Synonyms: Transcriptional activator GLI3,GLI3 form of 190 kDa,GLI3-190,GLI3 full length protein,GLI3FL,Transcriptional repressor GLI3R,GLI3 C-terminally truncated form,GLI3 form of 83 kDa,GLI3-83,GLI3,

    Tissue Specificity: Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.

    Background: GLI3 (Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

    Sequence Similarities: Belongs to the GLI C2H2-type zinc-finger protein family.

    Molekulargewicht
    33 kDa
    UniProt
    P10071
    Pathways
    Hedgehog Signalweg
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