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GLI3 Antikörper (N-Term)

Der Kaninchen Polyklonal Anti-GLI3-Antikörper wurde für WB validiert. Er ist geeignet, GLI3 in Proben von Human, Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.
Produktnummer ABIN3044156

Kurzübersicht für GLI3 Antikörper (N-Term) (ABIN3044156)

Target

Alle GLI3 Antikörper anzeigen
GLI3 (GLI Family Zinc Finger 3 (GLI3))

Reaktivität

  • 38
  • 16
  • 6
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 34
  • 5
  • 2
Kaninchen

Klonalität

  • 28
  • 13
Polyklonal

Konjugat

  • 27
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser GLI3 Antikörper ist unkonjugiert

Applikation

  • 21
  • 12
  • 11
  • 9
  • 7
  • 5
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Western Blotting (WB)
  • Bindungsspezifität

    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 40-57, N-Term

    Verwendungszweck

    Anti-Transcriptional activator GLI3 Gli3 Antibody Picoband®

    Sequenz

    SNEDESPGQT YHRERRNA

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Transcriptional activator GLI3 Gli3 Antibody (ABIN3044156). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human Gli3, different from the related rat sequence by two amino acids, and from the related mouse sequence by one amino acid.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
    1. Barna, M., Pandolfi, P. P., Niswander, L. Gli3 and Plzf cooperate in proximal limb patterning at early stages of limb development. (Letter) Nature 436: 277-281, 2005. 2. Bose, J., Grotewold, L., Ruther, U. Pallister-Hall syndrome phenotype in mice mutant for Gli3. Hum. Molec. Genet. 11: 1129-1135, 2002. 3. Craig, D. W., Itty, A., Panganiban, C., Szelinger, S., Kruer, M. C., Sekar, A., Reiman, D., Narayanan, V., Stephan, D. A., Kerrigan, J. F.Identification of somatic chromosomal abnormalities in hypothalamic hamartoma tissue at the GLI3 locus. Am. J. Hum. Genet. 82: 366-374, 2008.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    Konservierungsmittel

    Thimerosal (Merthiolate), Sodium azide

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Kumari, Chaurasia, Kumar, Dash: "Anti-apoptotic role of sonic hedgehog on blood platelets." in: Thrombosis research, Vol. 134, Issue 6, pp. 1311-5, (2014) (PubMed).

  • Target

    GLI3 (GLI Family Zinc Finger 3 (GLI3))

    Andere Bezeichnung

    GLI3

    Hintergrund

    Synonyms: Transcriptional activator GLI3,GLI3 form of 190 kDa,GLI3-190,GLI3 full length protein,GLI3FL,Transcriptional repressor GLI3R,GLI3 C-terminally truncated form,GLI3 form of 83 kDa,GLI3-83,GLI3,

    Tissue Specificity: Is expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium.

    Background: GLI3 (Gli-kruppel family member 3), also called ONCOGENE GLI3, encodes a member of the zinc finger gene family related to Kruppel, a gene that is known to regulate development in Drosophila. The GLI3 gene is expressed as an 8.5-kb mRNA in tissues such as testis, myometrium, placenta, and lung, and the protein product (relative molecular mass, 190,000) shows sequence-specific DNA binding. The GLI3 gene is mapped on 7p14.1. GLI3 is homologous to the Drosophila cubitus interruptus (ci) gene product (Ci), which regulates the Patched (pct), gooseberry (gsb), and decapentaplegic (dpp) genes. PKA-dependent processing of vertebrate GLI3 in developing limb generates a potent repressor in a manner antagonized by apparent long-range signaling from posteriorly localized Sonic hedgehog protein. The high relative abundance and potency of GLI3 repressor suggested specialization of GLI3 and its products for negative hedgehog pathway regulation.Coimmunoprecipitation and immunoblot studies showed that GLI3 protein is polyubiquitinated and that its processing depends on proteasome activity. The findings suggested that BTRC is required for GLI3 processing.

    Sequence Similarities: Belongs to the GLI C2H2-type zinc-finger protein family.

    Molekulargewicht

    33 kDa

    UniProt

    P10071

    Pathways

    Hedgehog Signalweg
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