MBD4 Antikörper (C-Term)

Produktnummer ABIN3044062

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MBD4 in WB. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für MBD4 Antikörper (C-Term) (ABIN3044062)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MBD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MBD4 Antikörper

Bindungsspezifität: AA 566-580, C-Term

Verwendungszweck: Anti-MBD4 Antibody Picoband®

Sequenz: YHDWLWENHE KLSLS

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-MBD4 Antibody (ABIN3044062). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MBD4, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Hendrich, B., Abbott, C., McQueen, H., Chambers, D., Cross, S., Bird, A. Genomic structure and chromosomal mapping of the murine and human Mbd1, Mbd2, Mbd3, and Mbd4 genes. Mammalian Genome 10: 906-912, 1999. 2. Hendrich, B., Bird, A. Identification and characterization of a family of mammalian methyl-CpG binding proteins. Molec. Cell. Biol. 18: 6538-6547, 1998. 3. Riccio, A., Aaltonen, L. A., Godwin, A. K., Loukola, A., Percesepe, A., Salovaara, R., Masciullo, V., Genuardi M., Paravatou-Petsotas, M., Bassi, D. E., Ruggeri, B. A., Klein-Szanto, A. J. P., Testa, J. R., Neri, G., Bellacosa, A. The DNA repair gene MBD4 (MED1) is mutated in human carcinomas with microsatellite instability. (Letter) Nature Genet. 23: 266-268, 1999.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu MBD4

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Andere Bezeichnung: MBD4

Hintergrund:

Synonyms: Methyl-CpG-binding domain protein 4,3.2.2.-,Methyl-CpG-binding endonuclease 1,Methyl-CpG-binding protein MBD4,Mismatch-specific DNA N-glycosylase,MBD4,MED1,

Tissue Specificity: Expressed in a wide variety of tissues but no or very low expression in brain and peripheral leukocytes. .

Background: MBD4 (Methyl-CpG-Binding Domain Protein 4), also known as MED1, is a protein that in humans is encoded by the MBD4 gene. MBD4 specifically binds methylated DNA, colocalizes with methylated sequences, and is likely to mediate the effects of DNA methylation in mammalian cells (Hendrich and Bird, 1998). Riccio et al. (1999) mapped the MBD4 gene to chromosome 3q21-q22 by FISH. Hendrich and Bird (1998) found that both MBD2 and MBD4 specifically bound methylated DNA in vitro and colocalized with methylated sequences in vivo. They concluded that MBD2 and MBD4 are likely to be mediators of the effects of DNA methylation in mammalian cells. Hendrich et al. (1999) showed that MBD4 contains a methyl-CpG-binding domain that can efficiently remove thymine or uracil from mismatched CpG sites in vitro. Furthermore, the methyl-CpG-binding domain of MBD4 binds preferentially to 5-methylcytosine CpG-TpG mismatches--the primary product of deamination at methyl-CpG.

Sequence Similarities: Contains 1 MBD (methyl-CpG-binding) domain.

Molekulargewicht: 80 kDa

UniProt: O95243

Pathways: DNA Reparatur