PDPK1 Antikörper (C-Term)

Produktnummer ABIN3044043

Produktdetails anti-PDPK1 Antikörper

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Bindungsspezifität: AA 408-421, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDPK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial(PDK1) detection. Tested with WB, IHC-P in Human,Rat.

Sequenz: WKHYNTNHEA DDWC

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial(PDK1) detection. Tested with WB, IHC-P in Human,Rat.
Gene Name: pyruvate dehydrogenase kinase, isozyme 1
Protein Name: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Mitochondrial Pyruvate dehydrogenase kinase 1(408-421aa WKHYNTNHEADDWC), different from the related rat sequence by one amino acid, and from the related mouse sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-PDPK1 Antikörper (ABIN3044043)

Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Xu, Ding, Fang, Zheng, Gao, Lu, Zhang: "Silencing of HIF-1alpha suppresses tumorigenicity of renal cell carcinoma through induction of apoptosis." in: Cancer gene therapy, Vol. 17, Issue 3, pp. 212-22, (2010) (PubMed).

Details zu PDPK1

Target: PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Andere Bezeichnung: PDK1 (PDPK1 Produkte)

Synonyme: pdpk1 antikoerper, MGC82080 antikoerper, PDK1 antikoerper, PDPK2 antikoerper, PRO0461 antikoerper, Pdk1 antikoerper, zgc:153787 antikoerper, 3'-phosphoinositide-dependent protein kinase 1 antikoerper, 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1 antikoerper, ATPDK1 antikoerper, AtPDK1 antikoerper, T32M21.110 antikoerper, T32M21_110 antikoerper, zgc:77318 antikoerper, 3-phosphoinositide dependent protein kinase 1 antikoerper, 3-phosphoinositide dependent protein kinase 1 L homeolog antikoerper, 3-phosphoinositide dependent protein kinase-1 antikoerper, 3-phosphoinositide dependent protein kinase 1a antikoerper, 3-phosphoinositide-dependent protein kinase 1 antikoerper, 3'-phosphoinositide-dependent protein kinase 1 antikoerper, 3-phosphoinositide dependent protein kinase 1b antikoerper, PDPK1 antikoerper, pdpk1.L antikoerper, pdpk1 antikoerper, Pdpk1 antikoerper, pdpk1a antikoerper, LOC100380750 antikoerper, pdk-1 antikoerper, PDK1 antikoerper, pdpk1b antikoerper

Hintergrund: PDK1(Pyruvate Dehydrogenase Kinase Isoenzyme 1), is an enzyme that in humans is encoded by the PDK1 gene. To find human PDKs, Gudi et al.(1995) used oligonucleotide primers to screen a human liver cDNA library by PCR. They identified and reported the deduced amino acid sequences of PDK1, PDK2, and PDK3. The human PDK1 gene encodes a protein with a predicted molecular mass of 49,244 Da that shares 93 % identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart.

Synonyms: [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1 antibody|[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial antibody|HGNC:8809 antibody|mitochondrial antibody|Mitochondrial pyruvate dehydrogenase kinase isoenzyme 1 antibody|PDH kinase 1 antibody|Pdk1 antibody|PDK1_HUMAN antibody|Pyruvate dehydrogenase kinase isoform 1 antibody|Pyruvate dehydrogenase kinase, isoenzyme 1 antibody

UniProt: Q15118

Pathways: PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals