NADPH Oxidase 4 Antikörper (C-Term)

Produktnummer ABIN3044011

Produktdetails anti-NADPH Oxidase 4 Antikörper

Target: NADPH Oxidase 4 (NOX4)

Bindungsspezifität: AA 561-578, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NADPH Oxidase 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for NADPH oxidase 4(NOX4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: NRNNSYGTKF EYNKES

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for NADPH oxidase 4(NOX4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: NADPH oxidase 4
Protein Name: NADPH oxidase 4

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse NADPH oxidase 4(561-578aa NRNNSYGTKFEYNKES), identical to the related rat sequence and different from the related human sequence by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-NADPH Oxidase 4 Antikörper (ABIN3044011)

Liu, Hong, Li, Ren, Wang, Xu, Shi, Xu: "A Cross Talk Between BRG1 and Males Absent on the First Contributes to Reactive Oxygen Species Production in a Mouse Model of Nonalcoholic Steatohepatitis." in: Antioxidants & redox signaling, (2018) (PubMed).

Meyer, Fredette, Daniel, Sharma, Amann, Arterburn, Barton, Prossnitz: "Obligatory role for GPER in cardiovascular aging and disease." in: Science signaling, Vol. 9, Issue 452, pp. ra105, (2017) (PubMed).

Details zu NADPH Oxidase 4

Target: NADPH Oxidase 4 (NOX4)

Andere Bezeichnung: NOX4 (NOX4 Produkte)

Hintergrund: NOX4(NADPH oxidase 4), also called RENAL NADPH OXIDASE or RENOX, is an enzyme that in humans is encoded by the NOX4 gene, and a member of the NOX family of NADPH oxidases. Geiszt et al.(2000) stated that the nucleotide sequence of RENOX matches that found in a genomic clone on chromosome 15. In a note added in proof, they stated that genomic clones assigned to chromosome 11 also contain sequence corresponding to RENOX. By FISH, Shiose et al.(2001) mapped the NOX4 gene to chromosome 11q14.2-q21. Geiszt et al.(2000) found that NIH 3T3 fibroblasts overexpressing transfected RENOX showed increased production of superoxide and developed signs of cellular senescence. They suggested that RENOX, as a renal source of ROS, may fulfill the function of the putative oxygen sensor in the kidney. By biochemical analysis of endogenous renal NOX4, Shiose et al.(2001) determined that the enzyme can use either NADH or NADPH as an electron donor for superoxide production.

Synonyms: Kidney oxidase-1 antibody|Kidney superoxide-producing NADPH oxidase antibody|KOX 1 antibody|KOX antibody|Kox-1 antibody|NADPH antibody|NADPH oxidase 4 antibody|Nox4 antibody|NOX4_HUMAN antibody|Renal NAD(P)H-oxidase antibody|RENOX antibody

Pathways: Carbohydrate Homeostasis, Smooth Muscle Cell Migration