Gelsolin Antikörper (C-Term)

Produktnummer ABIN3043978

Produktdetails anti-Gelsolin Antikörper

Target: Gelsolin (GSN)

Bindungsspezifität: AA 763-775, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Gelsolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Gelsolin(GSN) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Sequenz: WDDDYWSVDP LDR

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Gelsolin(GSN) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: gelsolin
Protein Name: Gelsolin

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Gelsolin(763-775aa WDDDYWSVDPLDR), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, The detection limit for GSN is approximately 1.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-Gelsolin Antikörper (ABIN3043978)

Guo, Cui, Jiang, Zhang, Jiang: "Proteomics of acute heart failure in a rat post-myocardial infarction model." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1946-1956, (2018) (PubMed).

Details zu Gelsolin

Target: Gelsolin (GSN)

Andere Bezeichnung: GSN (GSN Produkte)

Synonyme: ADF antikoerper, AGEL antikoerper, CG1106 antikoerper, DGS antikoerper, Dmel\\CG1106 antikoerper, gel antikoerper, scin antikoerper, cb107 antikoerper, gsn antikoerper, sb:cb107 antikoerper, u-gelsolin antikoerper, wu:fi16f06 antikoerper, gelsolin antikoerper, Gelsolin antikoerper, gelsolin S homeolog antikoerper, gelsolin a antikoerper, GSN antikoerper, Gel antikoerper, Gsn antikoerper, gsn.S antikoerper, gsn antikoerper, gsna antikoerper

Hintergrund: Gelsolin also known as GNS is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. Gelsolin is also known as brevin, or actin-depolymerizing factor, it is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

Synonyms: Actin depolymerizing factor antibody|Actin-depolymerizing factor antibody|ADF antibody|AGEL antibody|Amyloidosis Finnish type antibody|Brevin antibody|DKFZp313L0718 antibody|GELS_HUMAN antibody|Gelsolin antibody|GSN antibody

UniProt: P06396

Pathways: Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, Autophagie