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WISP1 Antikörper (C-Term)

Dieses Anti-WISP1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von WISP1 in WB und IHC. Geeignet für Human, Maus und Ratte.
Produktnummer ABIN3043974

Kurzübersicht für WISP1 Antikörper (C-Term) (ABIN3043974)

Target

Alle WISP1 Antikörper anzeigen
WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reaktivität

  • 47
  • 29
  • 4
  • 2
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 63
  • 6
  • 1
  • 1
Kaninchen

Klonalität

  • 66
  • 5
Polyklonal

Konjugat

  • 34
  • 11
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser WISP1 Antikörper ist unkonjugiert

Applikation

  • 45
  • 30
  • 21
  • 13
  • 13
  • 13
  • 10
  • 7
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 15
    • 7
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 354-367, C-Term

    Verwendungszweck

    Anti-WISP1 Antibody Picoband®

    Sequenz

    ADLESYPDFS EIAN

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-WISP1 Antibody (ABIN3043974). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human WISP1, different from the related mouse and rat sequences by one amino acid.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    1. Pennica, D., Swanson, T. A., Welsh, J. W., Roy, M. A., Lawrence, D. A., Lee, J., Brush, J., Taneyhill, L. A., Deuel, B., Lew, M., Watanabe, C., Cohen, R. L., Melhem, M. F., Finley, G. G., Quirke, P., Goddard, A. D., Hillan, K. J., Gurney, A. L., Botstein, D., Levine, A. J. WISP genes are members of the connective tissue growth factor family that are up-regulated in Wnt-1-transformed cells and aberrantly expressed in human colon tumors. Proc. Nat. Acad. Sci. 95: 14717-14722, 1998. 2. Tanaka, S., Sugimachi, K., Saeki, H., Kinoshita, J., Ohga, T., Shimada, M., Maehara, Y., Sugimachi, K. A novel variant of WISP1 lacking a von Willebrand type C module overexpressed in scirrhous gastric carcinoma. Oncogene 20: 5525-5532, 2001.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

    Andere Bezeichnung

    WISP1

    Hintergrund

    Synonyms: WNT1-inducible-signaling pathway protein 1,WISP-1,CCN family member 4,Wnt-1-induced secreted protein,WISP1,CCN4,

    Tissue Specificity: Expressed in heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. Isoform 2 is expressed predominantly in scirrhous gastric carcinoma and, weakly in placenta. Overexpression is associated with several cancers including breast cancer and colon tumors. Isoform 2 is overexpressed in scirrhous gastric carcinoma.

    Background: WISP1 (WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed (Tanaka et al., 2001). By use of radiation hybrid mapping panels, Pennica et al. (1998) mapped the WISP1 to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. Pennica et al. (1998) found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84 % of tumors examined compared with patient-matched normal mucosa.

    Sequence Similarities: Belongs to the CCN family.

    Molekulargewicht

    45 kDa

    UniProt

    O95388

    Pathways

    WNT Signalweg, Growth Factor Binding
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