EIF2AK2 Antikörper (C-Term)

Produktnummer ABIN3043824

Der Kaninchen Polyklonal anti-EIF2AK2 Antikörper (ABIN3043824) detektiert spezifisch EIF2AK2 in WB, IF, ICC und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für EIF2AK2 Antikörper (C-Term) (ABIN3043824)

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EIF2AK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Produktdetails anti-EIF2AK2 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: Anti-PKR/EIF2AK2 Antibody

Sequenz: EKTLLQKLLS KKPEDRPNTS EILRTLTVWK KSPEKNERHT C

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: different from the related mouse sequence by fifteen amino acids,and from the related rat sequence by thirteen amino acids.

Produktmerkmale: Anti-PKR/EIF2AK2 Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PKR, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by thirteen amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu EIF2AK2

Target: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2 (EIF2AK2))

Andere Bezeichnung: EIF2AK2

Hintergrund:

Background: EIF2AK2 (Eukaryotic Translation Initiation Factor 2-Alpha Kinase 2), also called PKR, is an enzyme that in humans is encoded by the EIF2AK2 gene. Activation of EIF2AK2 allows the kinase to phosphorylate its natural substrate, the alpha subunit of eukaryotic protein synthesis initiation factor-2, leading to the inhibition of protein synthesis. By FISH analysis, Squire et al. (1993) assigned the EIF2AK2 gene to the boundary between chromosome 2p22-p21. Ben-Asouli et al. (2002) showed that human gamma-interferon mRNA uses local activation of PKR in the cell to control its own translation yield. IFNG mRNA was found to activate PKR through a pseudoknot in its 5-prime untranslated region. Taylor et al. (1999) studied the mechanism underlying the resistance of hepatitis C virus (HCV) to interferon. They demonstrated that the HCV envelope protein E2 contains a sequence identical with phosphorylation sites of the interferon-inducible protein kinase PKR and the translation initiation factor EIF2-alpha, a target of PKR. E2 inhibited the kinase activity of PKR and blocked its inhibitory effect on protein synthesis and cell growth.

Gene Full Name: eukaryotic translation initiation factor 2 alpha kinase 2

Molekulargewicht: 69 kDa

Gen-ID: 5610

UniProt: P19525

Pathways: DNA Reparatur, ER-Nucleus Signaling, Hepatitis C