APEX1 Antikörper (AA 2-318)

Produktnummer ABIN3043785

Produktdetails anti-APEX1 Antikörper

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Bindungsspezifität: AA 2-318

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APEX1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase(APEX1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for DNA-(apurinic or apyrimidinic site) lyase(APEX1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: APEX nuclease (multifunctional DNA repair enzyme) 1
Protein Name: DNA-(apurinic or apyrimidinic site) lyase

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human APE1 recombinant protein (Position: P2-L318). Human APE1 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat APE1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for APE1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu APEX1

Target: APEX1 (Apurinic/Apyrimidinic Endonuclease 1 (APEX1))

Andere Bezeichnung: APEX1 (APEX1 Produkte)

Hintergrund: APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases.

Synonyms: AP endonuclease 1 antibody|AP endonuclease class I antibody|AP lyase antibody|APE 1 antibody|APE antibody|APE-1 antibody|APEN antibody|APEX 1 antibody|APEX antibody|APEX nuclease (multifunctional DNA repair enzyme) 1 antibody|Apex nuclease 1 antibody|Apex nuclease antibody|APEX1 antibody|APEX1_HUMAN antibody|Apurinic endonuclease antibody|Apurinic-apyrimidinic endonuclease 1 antibody|Apurinic/apyrimidinic (abasic) endonuclease antibody|Apurinic/apyrimidinic endonuclease 1 antibody|Apurinic/apyrimidinic exonuclease antibody|APX antibody|BAP1 antibody|Deoxyribonuclease (apurinic or apyrimidinic) antibody|DNA (apurinic or apyrimidinic site) lyase antibody|DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody|EC 4.2.99.18 antibody|HAP 1 antibody|HAP1 antibody|Human Apurinic endonuclease 1 antibody|MGC139790 antibody|Multifunctional DNA repair enzyme antibody|Redox factor 1 antibody|Redox factor-1 antibody|REF 1 antibody|REF 1 protein antibody|REF-1 antibody|REF1 antibody|REF1 protein antibody

Gen-ID: 328

UniProt: P27695

Pathways: DNA Reparatur, Chromatin Binding, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Positive Regulation of Response to DNA Damage Stimulus