AIM2 Antikörper (AA 14-215)
Kurzübersicht für AIM2 Antikörper (AA 14-215) (ABIN3043780)
Target
Alle AIM2 Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
-
-
Bindungsspezifität
- AA 14-215
-
Verwendungszweck
- Anti-AIM2 Antibody Picoband®
-
Kreuzreaktivität (Details)
- No cross-reactivity with other proteins
-
Produktmerkmale
- Anti-AIM2 Antibody Picoband® (ABIN3043780). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
-
Aufreinigung
- Immunogen affinity purified.
-
Immunogen
- E.coli-derived human AIM2 recombinant protein (Position: L14-H215). Human AIM2 shares 53.8% amino acid (aa) sequence identity with mouse AIM2.
-
Isotyp
- IgG
-
-
-
-
Applikationshinweise
-
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse
1. DeYoung KL, Ray ME, Su YA, Anzick SL, Johnstone RW, Trapani JA, Meltzer PS, Trent JM (Aug 1997). "Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma". Oncogene 15 (4): 453-7. 2. "Entrez Gene: AIM2 absent in melanoma 2". 3. Woerner SM, Kloor M, Schwitalle Y, Youmans H, von Knebel Doeberitz M, Gebert J, Dihlmann S (2007). "The putative tumor suppressor AIM2 is frequently affected by different genetic alterations in microsatellite unstable colon cancers". Genes Chromosomes Cancer 46 (12): 1080-9. -
Kommentare
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
-
Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
-
-
Format
- Lyophilized
-
Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
-
Konzentration
- 500 μg/mL
-
Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
-
Konservierungsmittel
- Sodium azide
-
Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Handhabung
- Avoid repeated freezing and thawing.
-
Lagerung
- 4 °C,-20 °C
-
Informationen zur Lagerung
-
Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
-
-
- AIM2 (Absent in Melanoma 2 (AIM2))
-
Andere Bezeichnung
- AIM2
-
Hintergrund
-
Synonyms: Interferon-inducible protein AIM2;Absent in melanoma 2;AIM2;
Tissue Specificity: Expressed in spleen, small intestine, peripheral blood leukocytes, and testis. .
Background: Interferon-inducible protein AIM2, also known as absent in melanoma 2 or simply AIM2, is a protein that in humans is encoded by the AIM2 gene. It is mapped to 1q22. AIM2 is a member of the Ifi202/IFI16 family. It plays a putative role in tumorigenic reversion and may control cell proliferation. Interferon-gamma induces expression of AIM2. Though there has been virtually no biochemistry performed, a model based on cell-based or in vivo experiments has led to the current model of how AIM2 triggers the inflammasome. The C-terminal HIN domain binds double stranded DNA (either viral, bacterial, or even host) and acts as a cytosolic dsDNA sensor. This leads to the oligomerization of the inflammasome complex. The N-terminal pyrin domain of AIM2 interacts with the pyrin domain of another protein ASC (or Apoptosis-associated Speck-like protein containing a caspase activation and recruitment domain). ASC also contains a CARD domain (caspase activation and recruitment domain), that recruits procaspase-1 to the complex. This leads to the autoactivation of caspase-1, an enzyme that processes proinflammatory cytokines (IL-1b and IL-18).
-
Molekulargewicht
- 45 kDa
-
Gen-ID
- 9447
-
UniProt
- O14862
-
Pathways
- Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
Target
-