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ATG14 Antikörper (N-Term)

Der Kaninchen Polyklonal Anti-ATG14-Antikörper wurde für WB, IHC, FACS, IF und ICC validiert. Er ist geeignet, ATG14 in Proben von Human und Ratte zu detektieren.
Produktnummer ABIN3043721

Kurzübersicht für ATG14 Antikörper (N-Term) (ABIN3043721)

Target

Alle ATG14 Antikörper anzeigen
ATG14 (ATG14 Autophagy Related 14 (ATG14))

Reaktivität

  • 35
  • 7
  • 6
  • 1
Human, Ratte

Wirt

  • 27
  • 8
Kaninchen

Klonalität

  • 27
  • 8
Polyklonal

Konjugat

  • 19
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser ATG14 Antikörper ist unkonjugiert

Applikation

  • 22
  • 19
  • 12
  • 6
  • 6
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • Bindungsspezifität

    • 7
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 70-101, N-Term

    Verwendungszweck

    Anti-ATG14L Antibody Picoband®

    Sequenz

    RDRERFIDKK ERLSRLKSKQ EEFQKEVLKA ME

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-ATG14L Antibody Picoband® (ABIN3043721). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the N-terminus of human ATG14L, different from the related mouse and rat sequences by two amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Immunofluorescence, 5 μg/mL, Human, Rat
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Diao J, Liu R, Rong Y, Zhao M, Zhang J, Lai Y, Zhou Q, Wilz LM, Li J, Vivona S, Pfuetzner RA, Brunger AT, Zhong Q. ""ATG14 promotes membrane tethering and fusion of autophagosomes to endolysosomes"". Nature, 2015 Apr 23.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    ATG14 (ATG14 Autophagy Related 14 (ATG14))

    Andere Bezeichnung

    ATG14

    Hintergrund

    Synonyms: Beclin 1-associated autophagy-related key regulator ,Barkor ,Autophagy-related protein 14-like protein ,Atg14L ,ATG14 ,ATG14L , KIAA0831,

    Tissue Specificity: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. .

    Background: ATG14 (also known as beclin-1-associated autophagy-related key regulator (Barkor) or ATG14L), an essential autophagy-specific regulator of the class III phosphatidylinositol 3-kinase complex, promotes membrane tethering of protein-free liposomes, and enhances hemifusion and full fusion of proteoliposomes reconstituted with the target (t)-SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) syntaxin 17 (STX17) and SNAP29, and the vesicle (v)-SNARE VAMP8 (vesicle-associated membrane protein 8). ATG14 binds to the SNARE core domain of STX17 through its coiled-coil domain, and stabilizes the STX17-SNAP29 binary t-SNARE complex on autophagosomes.

    Sequence Similarities: Belongs to the ATG14 family.

    Molekulargewicht

    59 kDa

    Gen-ID

    22863

    Pathways

    Autophagie
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