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PDPK1 Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch PDPK1 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.
Produktnummer ABIN3043600

Kurzübersicht für PDPK1 Antikörper (C-Term) (ABIN3043600)

Target

Alle PDPK1 Antikörper anzeigen
PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

Reaktivität

  • 179
  • 120
  • 82
  • 10
  • 6
  • 5
  • 5
  • 5
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 180
  • 22
  • 1
Kaninchen

Klonalität

  • 171
  • 32
Polyklonal

Konjugat

  • 100
  • 9
  • 8
  • 7
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 5
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
Dieser PDPK1 Antikörper ist unkonjugiert

Applikation

  • 159
  • 70
  • 53
  • 53
  • 44
  • 22
  • 16
  • 16
  • 12
  • 12
  • 7
  • 6
  • 3
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC)
  • Bindungsspezifität

    • 24
    • 19
    • 15
    • 15
    • 15
    • 15
    • 8
    • 7
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 524-556, C-Term

    Verwendungszweck

    Anti-PDPK1 Antibody Picoband®

    Sequenz

    YLMDPSGNAH KWCRKIQEVW RQRYQSHPDA AVQ

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins.

    Produktmerkmale

    Anti-PDPK1 Antibody Picoband® (ABIN3043600). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human PDPK1, different from the related mouse and rat sequences by two amino acids.

    Isotyp

    IgG
  • Applikationshinweise

    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
    Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
    Western blot, 0.1-0.5 μg/mL
    1. "Entrez Gene: PDPK1". 2. Mora A, Komander D, van Aalten DM, Alessi DR (April 2004). "PDK1, the master regulator of AGC kinase signal transduction". Semin. Cell Dev. Biol. 15 (2): 161-70. 3. Scortegagna M, Ruller C, Feng Y, Lazova R, Kluger H, Li JL, De SK, Rickert R, Pellecchia M, Bosenberg M, Ronai ZA (2014). "Genetic inactivation or pharmacological inhibition of Pdk1 delays development and inhibits metastasis of Braf(V600E)::Pten(-/-) melanoma". Oncogene 33(34): 4330-9.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    PDPK1 (3-phosphoinositide Dependent Protein Kinase-1 (PDPK1))

    Andere Bezeichnung

    PDPK1

    Hintergrund

    Synonyms: 3-phosphoinositide-dependent protein kinase 1,hPDK1,2.7.11.1,PDPK1,PDK1,

    Tissue Specificity: Appears to be expressed ubiquitously. The Tyr- 9 phosphorylated form is markedly increased in diseased tissue compared with normal tissue from lung, liver, colon and breast. .

    Background: 3-phosphoinositide dependent protein kinase-1, also known as PDPK1, is a protein which in humans is encoded by the PDPK1 gene. It is mapped to 16p13.3. PDPK1 is a master kinase, which is crucial for the activation of AKT/PKB and many other AGC kinases including PKC, S6K, SGK. An important role for PDPK1 is in the signalling pathways activated by several growth factors and hormones including insulin signaling. Mice lacking PDPK1 die during early embryonic development, indicating that this enzyme is critical for transmitting the growth-promoting signals necessary for normal mammalian development.

    Molekulargewicht

    70 kDa

    Gen-ID

    5170

    UniProt

    O15530

    Pathways

    PI3K-Akt Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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