RAD51 Antikörper (AA 1-258)

Produktnummer ABIN3043560

Dieses Anti-RAD51-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von RAD51 in WB. Geeignet für Human, Maus und Ratte.

Kurzübersicht für RAD51 Antikörper (AA 1-258) (ABIN3043560)

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD51 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RAD51 Antikörper

Bindungsspezifität: AA 1-258

Verwendungszweck: Anti-Rad51 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Rad51 Antibody Picoband® (ABIN3043560). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Rad51 recombinant protein (Position: M1-E258). Human Rad51 shares 98% amino acid (aa) sequence identity with mouse Rad51.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kato, M., Yano, K., Matsuo, F., Saito, H., Katagiri, T., Kurumizaka, H., Yoshimoto, M., Kasumi, F., Akiyama, F., Sakamoto, G., Nagawa, H., Nakamura, Y., Miki, Y. : Identification of Rad51 alteration in patients with bilateral breast cancer. J. Hum. Genet. 45: 133-137, 2000. 2. Daniel DC (2002). "Highlight: BRCA1 and BRCA2 proteins in breast cancer.". Microsc. Res. Tech. 59 (1): 68-83.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu RAD51

Target: RAD51 (DNA Repair Protein Homolog 1 (RAD51))

Andere Bezeichnung: RAD51

Hintergrund:

Synonyms: DNA repair protein RAD51 homolog 1,HsRAD51,hRAD51,RAD51 homolog A,RAD51,RAD51A, RECA,

Tissue Specificity: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

Background: DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene. The Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair. BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.

Sequence Similarities: Belongs to the RecA family. RAD51 subfamily.

Molekulargewicht: 37 kDa

Gen-ID: 5888

UniProt: Q06609

Pathways: DNA Reparatur