POLB Antikörper (AA 122-335)
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- Target Alle POLB Antikörper anzeigen
- POLB (Polymerase (DNA Directed), beta (POLB))
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Bindungsspezifität
- AA 122-335
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Reaktivität
- Human
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser POLB Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB)
- Verwendungszweck
- Rabbit IgG polyclonal antibody for DNA polymerase beta(POLB) detection. Tested with WB in Human.
- Kreuzreaktivität (Details)
- No cross reactivity with other proteins.
- Produktmerkmale
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Rabbit IgG polyclonal antibody for DNA polymerase beta(POLB) detection. Tested with WB in Human.
Gene Name: polymerase (DNA directed), beta
Protein Name: DNA polymerase beta - Aufreinigung
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human POLB recombinant protein (Position: L122-E335). Human POLB shares 95.3% and 94.9% amino acid (aa) sequence identity with mouse and rat POLB, respectively.
- Isotyp
- IgG
- Top Product
- Discover our top product POLB Primärantikörper
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- Applikationshinweise
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users. - Kommentare
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
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- Target
- POLB (Polymerase (DNA Directed), beta (POLB))
- Andere Bezeichnung
- POLB (POLB Produkte)
- Synonyme
- wu:fa97a05 antikoerper, zgc:109924 antikoerper, POLB antikoerper, DDBDRAFT_0186152 antikoerper, DDBDRAFT_0215784 antikoerper, DDBDRAFT_0232376 antikoerper, DDB_0186152 antikoerper, DDB_0215784 antikoerper, DDB_0232376 antikoerper, A430088C08Rik antikoerper, polymerase (DNA directed), beta antikoerper, DNA polymerase beta antikoerper, DNA polymerase X family protein antikoerper, polymerase (DNA directed), beta S homeolog antikoerper, polb antikoerper, POLB antikoerper, polB antikoerper, polb.S antikoerper, Polb antikoerper
- Hintergrund
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Polymerase (DNA directed), beta, also known as POLB, is an enzyme that, in humans, is encoded by the POLB gene. It is localized on 8p11.2. The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. It is found that a truncated POLB is expressed in primary colorectal tumors and inhibits the normal repair function of wildtype POLB. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. Additionally, human POLB forms a complex with and is methylated by PRMT6. In vitro, methylated POLB possesses significantly higher DNA polymerase activity when compared to that of unmodified enzyme. The increase in DNA polymerase activity upon methylation is due to the enhanced DNA binding and processivity of POLB.
Synonyms: DNA directed DNA polymerase beta antibody|DNA pol beta antibody|DNA polymerase beta antibody|DNA polymerase beta subunit antibody| DPOLB_HUMAN antibody|MGC125976 antibody|Pol B antibody|Pol beta antibody|POLB antibody|Polymerase (DNA directed) beta antibody - Gen-ID
- 5423
- UniProt
- P06746
- Pathways
- DNA Reparatur
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