POLB Antikörper (C-Term)

Produktnummer ABIN964928

Produktdetails anti-POLB Antikörper

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Bindungsspezifität: C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with overexpressed and endogenous POLß protein. A BLAST analysis was used to suggest reactivity with POLß from human, bovine, and Xenopus laevis based on a 100% homology with the immunizing sequence. Cross-reactivity with POLß from other sources has not been determined.

Produktmerkmale: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.

Aufreinigung: affinity purified

Sterilität: Sterile filtered

Immunogen: This affinity-purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptidecorresponding to a region near the C-terminus of the POLß protein.
Immunogen Type: Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody is suitable for ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38.1 kDa in size corresponding to POLß by western blotting in the appropriate cell lysate or extract.

Kommentare:

Gene Name: POLB

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.4 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Haltbarkeit: 12 months

Referenzen für anti-POLB Antikörper (ABIN964928)

Asagoshi, Liu, Masaoka, Lan, Prasad, Horton, Brown, Wang, Bdour, Sobol, Taylor, Yasui, Wilson: "DNA polymerase beta-dependent long patch base excision repair in living cells." in: DNA repair, Vol. 9, Issue 2, pp. 109-19, (2010) (PubMed).

Sobol, Foley, Nyska, Davidson, Wilson: "Regulated over-expression of DNA polymerase beta mediates early onset cataract in mice." in: DNA repair, Vol. 2, Issue 5, pp. 609-22, (2004) (PubMed).

Details zu POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Andere Bezeichnung: POLB (POLB Produkte)

Synonyme: wu:fa97a05 antikoerper, zgc:109924 antikoerper, POLB antikoerper, DDBDRAFT_0186152 antikoerper, DDBDRAFT_0215784 antikoerper, DDBDRAFT_0232376 antikoerper, DDB_0186152 antikoerper, DDB_0215784 antikoerper, DDB_0232376 antikoerper, A430088C08Rik antikoerper, polymerase (DNA directed), beta antikoerper, DNA polymerase beta antikoerper, DNA polymerase X family protein antikoerper, polymerase (DNA directed), beta S homeolog antikoerper, polb antikoerper, POLB antikoerper, polB antikoerper, polb.S antikoerper, Polb antikoerper

Hintergrund: The POLß protein belongs to the DNA polymerase type-X family. It is a DNA polymerase involved in gap-filling DNA synthesis or base excision and repair in a stepwise distributive fashion unlike other DNA polymerases. It is required for DNA maintenance, replication, recombination, and drug resistance. It is normally found as a monomer, in the cytoplasm and it translocates to the nucleus upon DNA damage.
Synonyms: DNA polymerase beta, POLB, beta-pol

Gen-ID: 5423

NCBI Accession: NP_002681

UniProt: P06746

Pathways: DNA Reparatur