ABI1 Antikörper (N-Term)

Produktnummer ABIN3043515

Produktdetails anti-ABI1 Antikörper

Target: ABI1 (Abl-Interactor 1 (ABI1))

Bindungsspezifität: AA 151-175, N-Term

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ABI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Verwendungszweck: Anti-SSH3BP1/ABI1 Antibody Picoband®

Sequenz: HGVKWLKAKH GNNQPARTGT LSRTN

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-SSH3BP1/ABI1 Antibody Picoband® (ABIN3043515). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ABI1, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Tani, K., Sato, S., Sukezane, T., Kojima, H., Hirose, H., Hanafusa, H., Shishido, T. : Abl interactor 1 promotes tyrosine 296 phosphorylation of mammalian Enabled (Mena) by c-Abl kinase. J. Biol. Chem. 278: 21685-21692, 2003. 2. Taki, T., Shibuya, N., Taniwaki, M., Hanada, R., Morishita, K., Bessho, F., Yanagisawa, M., Hayashi, Y. : ABI-1, a human homolog to mouse Abl-interactor 1, fuses the MLL gene in acute myeloid leukemia with t(10,11)(p11.2,q23). Blood 92: 1125-1130, 1998.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-ABI1 Antikörper (ABIN3043515)

Cui, Yu, Dong, Chen, Zhang, Liu: "Downregulation of ABI1 expression affects the progression and prognosis of human gastric carcinoma." in: Medical oncology (Northwood, London, England), Vol. 27, Issue 3, pp. 632-9, (2010) (PubMed).

Details zu ABI1

Target: ABI1 (Abl-Interactor 1 (ABI1))

Andere Bezeichnung: ABI1 (ABI1 Produkte)

Hintergrund:

Synonyms: Abl interactor 1,Abelson interactor 1,Abi-1,Abl-binding protein 4,AblBP4,Eps8 SH3 domain-binding protein,Eps8-binding protein,Nap1-binding protein,Nap1BP,Spectrin SH3 domain-binding protein 1,e3B1,ABI1,SSH3BP1,

Tissue Specificity: Widely expressed, with highest expression in brain. .

Background: SSH3BP1 is also known as ABI1. ABI1 is a human homolog of mouse Abl-interactor-1 (Abi1), mapped on 10p11.2. ABI1 participates in the transduction of signals from Ras to Rac by regulating Rac-specific guanine nucleotide exchange factor (GEF) activities. It dramatically promoted ABL1-mediated tyrosine phosphorylation of MENA, but not of other substrates. Abi-1 regulates c-Abl-mediated phosphorylation of Mena by interacting with both proteins. ABI1 plays a role in the leukemogenesis by translocating to MLL.

Sequence Similarities: Belongs to the ABI family.

Molekulargewicht: 65 kDa

Gen-ID: 10006

Pathways: RTK Signalweg, Response to Water Deprivation, ER-Nucleus Signaling