Cyclin B1 Antikörper (AA 1-433)

Produktnummer ABIN3043482

Dieses Anti-Cyclin B1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Cyclin B1 in WB, IF, FACS und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 17+ Publikationen zitiert.

Kurzübersicht für Cyclin B1 Antikörper (AA 1-433) (ABIN3043482)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Produktdetails anti-Cyclin B1 Antikörper

Bindungsspezifität: AA 1-433

Verwendungszweck: Anti-Cyclin B1/CCNB1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Cyclin B1/CCNB1 Antibody Picoband® (ABIN3043482). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cyclin B1 recombinant protein (Position: M1-V433). Human Cyclin B1 shares 86% and 85% amino acid (aa) sequences identity with mouse and rat Cyclin B1, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Sartor H, Ehlert F, Grzeschik KH, Muller R, Adolph S (Aug 1992). "Assignment of two human cell cycle genes, CDC25C and CCNB1, to 5q31 and 5q12, respectively". Genomics 13 (3): 911-2. 2. Kimura K, Hirano M, Kobayashi R, Hirano T (October 1998). "Phosphorylation and activation of 13S condensin by Cdc2 in vitro". Science 282 (5388): 487-90. 3. Heald R, McKeon F (May 1990). "Mutations of phosphorylation sites in lamin A that prevent nuclear lamina disassembly in mitosis". Cell 61 (4): 579-89.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Cyclin B1 Antikörper (ABIN3043482)

Guo, Guo, Zhao, Cai: "Active targeting co-delivery system based on hollow mesoporous silica nanoparticles for antitumor therapy in ovarian cancer stem-like cells." in: Oncology reports, Vol. 38, Issue 3, pp. 1442-1450, (2018) (PubMed).

Chen, Zhou, Yao, Dai, Zhou, Wang, Zhang: "Dipalmitoylphosphatidic acid inhibits breast cancer growth by suppressing angiogenesis via inhibition of the CUX1/FGF1/HGF signalling pathway." in: Journal of cellular and molecular medicine, Vol. 22, Issue 10, pp. 4760-4770, (2018) (PubMed).

Xiao, Dong, Cheng, Mao, Zong, Wu, Wang, Xie, Wang, Lei, Guo: "LRIG2 promotes the proliferation and cell cycle progression of glioblastoma cells in vitro and in vivo through enhancing PDGFRβ signaling." in: International journal of oncology, Vol. 53, Issue 3, pp. 1069-1082, (2018) (PubMed).

Yan, Zhang, Jin, Cai, Jia, Liu, Wang, Li, Zhang, Huang, Lai, Wang, Liu, Zeng, Cai, Jiang, Bai: "mTORC1 regulates PTHrP to coordinate chondrocyte growth, proliferation and differentiation." in: Nature communications, Vol. 7, pp. 11151, (2016) (PubMed).

Yin, Jiang, Peng, Cui, Zhou, He, Zuo, Ouyang, Fan, Fang: "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." in: Oncotarget, Vol. 7, Issue 24, pp. 35592-35606, (2016) (PubMed).

Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, (2015) (PubMed).

Zheng, Liu, Liu, Ma, Zhou, Chen, Chang, Wang, Yang, He: "Cucurbitacin B inhibits growth and induces apoptosis through the JAK2/STAT3 and MAPK pathways in SH?SY5Y human neuroblastoma cells." in: Molecular medicine reports, Vol. 10, Issue 1, pp. 89-94, (2014) (PubMed).

Zhu, Liu, Li, Liu, Wang, Sun, Xiong, Jiang, Zheng, Hu: "Protein tyrosine phosphatase receptor U (PTPRU) is required for glioma growth and motility." in: Carcinogenesis, Vol. 35, Issue 8, pp. 1901-10, (2014) (PubMed).

Liu, Ma, Wu, Li, Zhang, Han, Wang, Reindl, Wu, Ma: "Arginine deiminase augments the chemosensitivity of argininosuccinate synthetase-deficient pancreatic cancer cells to gemcitabine via inhibition of NF-?B signaling." in: BMC cancer, Vol. 14, pp. 686, (2014) (PubMed).

Liu, Zhu, Xiong, Zheng, Hu, Qiu: "Knockdown of protein tyrosine phosphatase receptor U inhibits growth and motility of gastric cancer cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 9, pp. 5750-61, (2014) (PubMed).

Ling, Lu, He, Xiao, Jiang, Su: "Diallyl disulfide selectively causes checkpoint kinase-1 mediated G2/M arrest in human MGC803 gastric cancer cell line." in: Oncology reports, Vol. 32, Issue 5, pp. 2274-82, (2014) (PubMed).

Li, Zhu, Liu, Liu, Wang, Xiong, Shen, Hu, Zheng: "ZFX knockdown inhibits growth and migration of non-small cell lung carcinoma cell line H1299." in: International journal of clinical and experimental pathology, Vol. 6, Issue 11, pp. 2460-7, (2013) (PubMed).

Rasul, Khan, Yu, Ali, Bo, Yang, Ma: "Isoalantolactone, a sesquiterpene lactone, induces apoptosis in SGC-7901 cells via mitochondrial and phosphatidylinositol 3-kinase/Akt signaling pathways." in: Archives of pharmacal research, Vol. 36, Issue 10, pp. 1262-9, (2013) (PubMed).

Yang, Gu, Zheng, Li, Lian, Miao, Jiang, Wei: "RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells." in: The Journal of biological chemistry, Vol. 286, Issue 13, pp. 11865-74, (2011) (PubMed).

Lin, Fu, Zhao, Lin, Zou, Liu, Zhu, Wang, Yu: "Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells." in: Molecular biology reports, Vol. 38, Issue 3, pp. 1741-7, (2011) (PubMed).

Ren, Wang, Shi, Yue, Zhang, Lei: "Effects of maternal and dietary selenium (Se-enriched yeast) on the expression of p34(cdc2) and CyclinB1 of germ cells of their offspring in goats." in: Animal reproduction science, Vol. 123, Issue 3-4, pp. 187-91, (2011) (PubMed).

Ye, Chen, Chen, Xie, Yang, Lou, Yu: "XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 133-41, (2009) (PubMed).

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: CCNB

Hintergrund:

Synonyms: G2/mitotic-specific cyclin-B1,CCNB1,CCNB,

Background: CCNB also known as Cyclin B1, is a protein that in humans is encoded by the CCNB1 gene, and it is mapped to 5q13.2. The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. CCNB contributes to the switch-like all or none behavior of the cell in deciding to commit to mitosis. Its activation is well-regulated, and positive feedback loops ensure that once the cyclin B1-Cdk1 complex is activated, it is not deactivated.

Sequence Similarities: Belongs to the cyclin family. Cyclin AB subfamily.

Molekulargewicht: 56 kDa

Gen-ID: 891

UniProt: P14635

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase