CAPNL1 Antikörper (AA 396-555)

Produktnummer ABIN3043467

Dieses Anti-CAPNL1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CAPNL1 in WB, IHC, ICC, IF und FACS. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 3+ Publikationen zitiert.

Kurzübersicht für CAPNL1 Antikörper (AA 396-555) (ABIN3043467)

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CAPNL1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-CAPNL1 Antikörper

Bindungsspezifität: AA 396-555

Verwendungszweck: Anti-Calpain 1/CAPN1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Calpain 1/CAPN1 Antibody Picoband® (ABIN3043467). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Smith IJ, et al. Calpain activity is increased in skeletal muscle from gastric cancer patients with no or minimal weight loss. Muscle Nerve, 2011 Mar. 2. Wang JC, et al. Proteolysis by endogenous calpain I leads to the activation of calcineurin in human heart. Clin Lab, 2012.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-CAPNL1 Antikörper (ABIN3043467)

Ye, Wang, Zhang, Song, Yang, Li, Li, Su, Yang, Ma: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).

Chen, Tang, Gao, Xu, Jiang, Cao, Fu, Sun, Liu, Shi: "Fibrosis, adipogenesis, and muscle atrophy in congenital muscular torticollis." in: Medicine, Vol. 93, Issue 23, pp. e138, (2014) (PubMed).

Mo, Chen, Li, Zheng, Ke, Deng, Li, Jiang, Wu, Wang, Wang, Yang, Cao: "Suppression of NHE1 by small interfering RNA inhibits HIF-1?-induced angiogenesis in vitro via modulation of calpain activity." in: Microvascular research, Vol. 81, Issue 2, pp. 160-8, (2011) (PubMed).

Details zu CAPNL1

Target: CAPNL1 (Calpain 1, Large Subunit (CAPNL1))

Andere Bezeichnung: CAPN1

Hintergrund:

Synonyms: Calpain-1 catalytic subunit,3.4.22.52,Calcium-activated neutral proteinase 1,CANP 1,Calpain mu-type,Calpain-1 large subunit,Cell proliferation-inducing gene 30 protein,Micromolar-calpain,muCANP,CAPN1,CANPL1,PIG30,

Tissue Specificity: Ubiquitous.

Background: CAPN1 is also known as CANP or muCL. The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.

Sequence Similarities: Belongs to the peptidase C2 family.

Molekulargewicht: 82 kDa

Gen-ID: 823

UniProt: P07384

Pathways: Apoptose