PKM Antikörper (N-Term)

Produktnummer ABIN3043381

Produktdetails anti-PKM Antikörper

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Bindungsspezifität: AA 475-500, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Pyruvate kinase PKM(PKM) detection. Tested with WB, IHC-P in Human.

Sequenz: KDPVQEAWAE DVDLRVNFAM NVGKAR

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Pyruvate kinase PKM(PKM) detection. Tested with WB, IHC-P in Human.
Gene Name: pyruvate kinase, muscle
Protein Name: Pyruvate kinase PKM

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PKM2(475-500aa KDPVQEAWAEDVDLRVNFAMNVGKAR), different from the related mouse sequence by five amino acids, and from the related rat sequence by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for PKM2 is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Andere Bezeichnung: PKM (PKM Produkte)

Synonyme: CTHBP antikoerper, OIP3 antikoerper, PK3 antikoerper, PKM2 antikoerper, TCB antikoerper, THBP1 antikoerper, AA414905 antikoerper, AL024370 antikoerper, AL024424 antikoerper, Pk-2 antikoerper, Pk-3 antikoerper, Pk3 antikoerper, Pkm2 antikoerper, PKM12 antikoerper, CG7070 antikoerper, Dmel\\CG7070 antikoerper, PYK antikoerper, Pyk antikoerper, cb1029 antikoerper, fj33f03 antikoerper, pkm2 antikoerper, pkm2a antikoerper, wu:fj33f03 antikoerper, MGC53388 antikoerper, PKM antikoerper, PK-S antikoerper, pk3 antikoerper, pkm2b antikoerper, zgc:92037 antikoerper, pyruvate kinase M1/2 antikoerper, pyruvate kinase, muscle antikoerper, Pyruvate kinase antikoerper, pyruvate kinase M1/2a antikoerper, pyruvate kinase PKM antikoerper, pyruvate kinase (PK) antikoerper, pyruvate kinase antikoerper, pyruvate kinase M1/2 S homeolog antikoerper, pyruvate kinase M1/2b antikoerper, PKM antikoerper, Pkm antikoerper, PyK antikoerper, pkma antikoerper, pkm antikoerper, LOC710798 antikoerper, pyk antikoerper, pk antikoerper, LOC100534543 antikoerper, pkm.S antikoerper, pkmb antikoerper

Hintergrund: PKM (Pyruvate Kinase, Muscle), also known as PK3 or PKM2, is an enzyme that in humans is encoded by the PKM gene. The activity of pyruvate kinase subtype M2 is increased by fructose 1, 6-bisphosphate (Fru-1, 6-P2). By in situ hybridization, Popescu and Cheng (1990) mapped the THBP1 gene to 15q24-q25. Ashizawa et al. (1991) manipulated the intracellular Fru-1, 6-P2 concentration in several mammalian cell lines, including human, by varying the glucose concentration in the media. Using a novel proteomic screen for phosphotyrosine-binding proteins, Christofk et al. (2008) observed that PKM2 binds directly and selectively to tyrosine-phosphorylated peptides.

Synonyms: CTHBP antibody|Cytosolic thyroid hormone binding protein antibody|Cytosolic thyroid hormone-binding protein antibody|KPYM_HUMAN antibody|MGC3932 antibody|OIP 3 antibody|OIP-3 antibody|Oip3 antibody|OIP3 antibody|OPA interacting protein 3 antibody|Opa-interacting protein 3 antibody|p58 antibody|PK Muscle type antibody|PK, muscle type antibody|PK2 antibody|Pk3 antibody|PKM antibody|PKM2 antibody|PYKM antibody|Pyruvate kinase 2/3 antibody|Pyruvate kinase 3 antibody|Pyruvate kinase isozymes M1/M2 antibody|Pyruvate kinase muscle antibody|Pyruvate kinase muscle isozyme antibody|Pyruvate kinase, muscle 2 antibody|TCB antibody|THBP1 antibody|Thyroid hormone binding protein 1 antibody|Thyroid hormone binding protein cytosolic antibody|Thyroid hormone-binding protein 1 antibody|Tumor M2 PK antibody|Tumor M2-PK antibody

Gen-ID: 5315

UniProt: P14618

Pathways: Warburg Effekt