PAX6 Antikörper (Middle Region)

Produktnummer ABIN3043379

Produktdetails anti-PAX6 Antikörper

Target: PAX6 (Paired Box 6 (PAX6))

Bindungsspezifität: AA 195-233, Middle Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAX6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-PAX6 Antibody Picoband®

Sequenz: EDSDEAQMRL QLKRKLQRNR TSFTQEQIEA LEKEFERTH

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-PAX6 Antibody Picoband® (ABIN3043379). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PAX6, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Gehring WJ, Ikeo K (September 1999). "Pax 6: mastering eye morphogenesis and eye evolution". Trends Genet. 15 (9): 371-7. 2. Jordan T, Hanson I, Zaletayev D, Hodgson S, Prosser J, Seawright A, Hastie N, van Heyningen V (August 1992). "The human PAX6 gene is mutated in two patients with aniridia". Nat. Genet. 1 (5): 328-32. 3. Walcher T, Xie Q, Sun J, Irmler M, Beckers J, 79ztürk T, Niessing D, Stoykova A, Cvekl A, Ninkovic J, Götz M (March 2013). "Functional dissection of the paired domain of Pax6 reveals molecular mechanisms of coordinating neurogenesis and proliferation". Development 140 (5): 1123-36.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-PAX6 Antikörper (ABIN3043379)

Liu, Novak, Pepin, Maschhoff, Worner, Chen, Zhang, Hu: "A congenital hydrocephalus-causing mutation in Trim71 induces stem cell defects via inhibiting Lsd1 mRNA translation." in: EMBO reports, Vol. 24, Issue 2, pp. e55843, (2023) (PubMed).

Details zu PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Andere Bezeichnung: PAX6 (PAX6 Produkte)

Hintergrund:

Synonyms: Paired box protein Pax-6,Aniridia type II protein,Oculorhombin,PAX6,AN2,

Tissue Specificity: Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.

Background: Paired box protein Pax-6, also known as aniridia type II protein (AN2) or oculorhombin, is a protein that in humans is encoded by the PAX6 gene. This gene encodes a homeobox and paired domain-containing protein that binds DNA and functions as a regulator of transcription. Activity of this protein is key in the development of neural tissues, particularly the eye. In addition, this gene is regulated by multiple enhancers located up to hundreds of kilobases distant from this locus. Mutations in this gene or in the enhancer regions can cause ocular disorders such as aniridia and Peter's anomaly. Use of alternate promoters and alternative splicing result in multiple transcript variants encoding different isoforms.

Molekulargewicht: 47 kDa

Gen-ID: 5080

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis