RAC1 Antikörper (C-Term)

Produktnummer ABIN3043366

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RAC1 in WB, IF und ICC. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für RAC1 Antikörper (C-Term) (ABIN3043366)

Target: RAC1

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-RAC1 Antikörper

Bindungsspezifität: AA 169-189, C-Term

Verwendungszweck: Anti-Rac1 Antibody Picoband®

Sequenz: FDEAIRAVLC PPPVKKRKRK C

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Rac1 Antibody Picoband® (ABIN3043366). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Rac1, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Didsbury J, Weber RF, Bokoch GM, Evans T, Snyderman R (October 1989). "rac, a novel ras-related family of proteins that are botulinum toxin substrates". J. Biol. Chem. 264 (28): 16378-82. 2. Jordan P, Brazåo R, Boavida MG, Gespach C, Chastre E (November 1999). "Cloning of a novel human Rac1b splice variant with increased expression in colorectal tumors".Oncogene 18 (48): 6835-9. 3. Zhou C, Licciulli S, Avila JL, Cho M, Troutman S, Jiang P, Kossenkov AV, Showe LC, Liu Q, Vachani A, Albelda SM, Kissil JL (February 2013). "The Rac1 splice form Rac1b promotes K-ras-induced lung tumorigenesis". Oncogene 32 (7): 903-9.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-RAC1 Antikörper (ABIN3043366)

Han, Zhuang, Chen, Zhu, Cai, Lu et al.: "Novel derivative of Paeonol, Paeononlsilatie sodium, alleviates behavioral damage and hippocampal dendritic injury in Alzheimer's disease concurrent with cofilin1/phosphorylated-cofilin1 and ..." in: PLoS ONE, Vol. 12, Issue 9, pp. e0185102, (2017) (PubMed).

Shen, Yang, Yao, Sun, Chen, Mo, Shi, Chen, Liu: "Gadolinium promoted proliferation in mouse embryo fibroblast NIH3T3 cells through Rac and PI3K/Akt signaling pathways." in: Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine, Vol. 27, Issue 4, pp. 753-62, (2015) (PubMed).

Details zu RAC1

Target: RAC1

Hintergrund:

Synonyms: Ras-related C3 botulinum toxin substrate 1,Cell migration-inducing gene 5 protein,Ras-like protein TC25,p21-Rac1,RAC1,TC25,MIG5,

Tissue Specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

Background: Rac1, also known as Ras-related C3 botulinum toxin substrate 1, is a protein found in human cells. It is encoded by the RAC1gene. This gene can produce a variety of alternatively splicedversions of the Rac1 protein, which appear to carry out different functions. This gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the small GTPase superfamily. Rho family.

Molekulargewicht: 21 kDa

Gen-ID: 5879

UniProt: P63000

Pathways: WNT Signalweg, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling