IL-3 Antikörper (Middle Region)

Produktnummer ABIN3043064

Der Kaninchen Polyklonal Anti-IL-3-Antikörper wurde für WB validiert. Er ist geeignet, IL-3 in Proben von Maus zu detektieren.

Kurzübersicht für IL-3 Antikörper (Middle Region) (ABIN3043064)

Target: IL-3 (Interleukin 3 (IL-3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL-3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-IL-3 Antikörper

Bindungsspezifität: AA 122-140, Middle Region

Verwendungszweck: Anti-Interleukin-3 IL3 Antibody Picoband®

Sequenz: RDLDDFRKKL RFYMVHLND

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Interleukin-3 IL3 Antibody (ABIN3043064). Tested in WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse IL-3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse
1. Le Beau, M. M., Epstein, N. D., O'Brien, S. J., Nienhuis, A. W., Yang, Y.-C., Clark, S. C., Rowley, J. D. The interleukin 3 gene is located on human chromosome 5 and is deleted in myeloid leukemias with a deletion of 5q. Proc. Nat. Acad. Sci. 84: 5913-5917, 1987. 2. Clark-Lewis, I., Aebersold, R., Ziltener, H., Schrader, J. W., Hood, L. E., Kent, S. B. H. Automated chemical synthesis of a protein growth factor for hemopoietic cells, interleukin-3. Science 231: 134-139, 1986. 3. Fung, M. C., Hapel, A. J., Ymer, S., Cohen, D. R., Johnson, R. M., Campbell, H. D., Young, I. G. Molecular cloning of cDNA for murine interleukin-3. Nature 307: 233-237, 1984. 4. Ihle, J. N., Silver, J., Kozak, C. A. Genetic mapping of the mouse interleukin 3 gene to chromosome 11. J. Immun. 138: 3051-3054, 1987. 5. Yang, Y.-C., Kovacic, S., Kriz, R., Wolf, S., Clark, S. C., Wellems, T. E., Nienhuis, A., Epstein, N. The human genes for GM-CSF and IL3 are closely linked in tandem on chromosome 5. Blood 71: 958-961, 1988.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Andere Bezeichnung: IL3

Hintergrund:

Synonyms: Interleukin-3,IL-3,Hematopoietic growth factor,Mast cell growth factor,MCGF,Multipotential colony-stimulating factor,P-cell-stimulating factor,Il3,Csfmu, Il-3,

Tissue Specificity: Activated T-cells, mast cells, natural killer cells.

Background: Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. Ot is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Molekulargewicht: 14 kDa

UniProt: P01586

Pathways: JAK-STAT Signalweg, Regulation of Carbohydrate Metabolic Process, Autophagie