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GLI2 Antikörper (C-Term)

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GLI2 in WB. Er zeigt eine Reaktivität gegenüber Human und wurde in 1 Publikation erwähnt.
Produktnummer ABIN3042988

Kurzübersicht für GLI2 Antikörper (C-Term) (ABIN3042988)

Target

Alle GLI2 Antikörper anzeigen
GLI2 (GLI Family Zinc Finger 2 (GLI2))

Reaktivität

  • 27
  • 13
  • 6
  • 1
Human

Wirt

  • 28
  • 2
Kaninchen

Klonalität

  • 28
  • 2
Polyklonal

Konjugat

  • 20
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser GLI2 Antikörper ist unkonjugiert

Applikation

  • 18
  • 12
  • 12
  • 6
  • 4
  • 3
  • 2
  • 1
Western Blotting (WB)
  • Bindungsspezifität

    • 5
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1444-1463, C-Term

    Verwendungszweck

    Anti-Zinc finger protein GLI2 Gli2 Antibody Picoband®

    Sequenz

    MLYYYGQIHM YEQDGGLENL

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Zinc finger protein GLI2 Gli2 Antibody (ABIN3042988). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human
    1. Bertolacini, C. D. P., Ribeiro-Bicudo, L. A., Petrin, A., Richieri-Costa, A., Murray, J. C. Clinical findings in patients with GLI2 mutations--phenotypic variability. Clin. Genet. 81: 70-75, 2012. 2. Matsumoto, N., Fujimoto, M., Kato, R., Niikawa, N. Assignment of the human GLI2 gene to 2q14 by fluorescence in situ hybridization. Genomics 36: 220-221, 1996. 3. Roessler, E., Ermilov, A. N., Grange, D. K., Wang, A., Grachtchouk, M., Dlugosz, A. A., Muenke, M. A previously unidentified amino-terminal domain regulates transcriptional activity of wild-type and disease-associated human GLI2. Hum. Molec. Genet. 14: 2181-2188, 2005.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

    Konservierungsmittel

    Thimerosal (Merthiolate), Sodium azide

    Vorsichtsmaßnahmen

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Xiang, Jiang, Guo, Gong, Yang, Wu, Huang, Cheng, Xu: "Hedgehog pathway inhibitor-4 suppresses malignant properties of chondrosarcoma cells by disturbing tumor ciliogenesis." in: Oncology reports, Vol. 32, Issue 4, pp. 1622-30, (2014) (PubMed).

  • Target

    GLI2 (GLI Family Zinc Finger 2 (GLI2))

    Andere Bezeichnung

    GLI2

    Hintergrund

    Synonyms: Zinc finger protein GLI2 ,GLI family zinc finger protein 2 ,Tax helper protein ,GLI2 ,THP ,

    Tissue Specificity: Isoform 1 and isoform 4 are expressed in HTLV- 1-infected T-cell lines (at protein level). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines. .

    Background: GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes, thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. (1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. (2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

    Sequence Similarities: Belongs to the GLI C2H2-type zinc-finger protein family.

    Molekulargewicht

    89 kDa

    UniProt

    P10070

    Pathways

    Hedgehog Signalweg, Dopaminergic Neurogenesis
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