BID Antikörper (C-Term)

Produktnummer ABIN3042950

Produktdetails anti-BID Antikörper

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Bindungsspezifität: AA 179-195, C-Term

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BID Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for BH3-interacting domain death agonist(Bid) detection. Tested with WB, IHC-P in Mouse,Rat.

Sequenz: NQNLFSYVRN LVRNEMD

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for BH3-interacting domain death agonist(Bid) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: BH3 interacting domain death agonist
Protein Name: BH3-interacting domain death agonist

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid(179-195aa NQNLFSYVRNLVRNEMD), different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Predicted Species: Rat, The detection limit for BID is approximately 2.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-BID Antikörper (ABIN3042950)

Wang, Liu, Mi, Wang, Tang, Zhao, Li: "Sonodynamically induced apoptosis by protoporphyrin IX on hepatoma-22 cells in vitro." in: Ultrasound in medicine & biology, Vol. 36, Issue 4, pp. 667-76, (2010) (PubMed).

Details zu BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Andere Bezeichnung: Bid (BID Produkte)

Synonyme: FP497 antikoerper, 2700049M22Rik antikoerper, AI875481 antikoerper, AU022477 antikoerper, BID antikoerper, bid antikoerper, si:ch211-238n5.6 antikoerper, fp497 antikoerper, xbid antikoerper, DKFZp469E066 antikoerper, BH3 interacting domain death agonist antikoerper, BH3 interacting domain death agonist S homeolog antikoerper, BID antikoerper, Bid antikoerper, bida antikoerper, bid.S antikoerper, bid antikoerper

Hintergrund: BID(BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al.(1998) mapped the human BID gene to 22q11. Luo et al.(1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Synonyms: Apoptic death agonist antibody|Apoptotic death agonist antibody|Apoptotic death agonist BID antibody|BH3 interacting domain death agonist antibody|BH3 interacting domain death agonist p11 antibody|BH3 interacting domain death agonist p13 antibody|BH3 interacting domain death agonist p15 antibody|BH3-interacting domain death agonist p11 antibody|BID antibody|BID isoform ES(1b) antibody|BID isoform L(2) antibody|BID isoform Si6 antibody|BID_HUMAN antibody|Desmocollin type 4 antibody|FP497 antibody|HGNC:1050 antibody|Human BID coding sequence antibody|MGC15319 antibody|MGC42355 antibody|p11 BID antibody|p13 BID antibody|p15 BID antibody|p22 BID antibody

UniProt: P70444

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity