BID Antikörper (C-Term)

Produktnummer ABIN3042950

Der Kaninchen Polyklonal Anti-BID-Antikörper wurde für WB, IHC und FACS validiert. Er ist geeignet, BID in Proben von Maus und Ratte zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für BID Antikörper (C-Term) (ABIN3042950)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BID Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-BID Antikörper

Bindungsspezifität: AA 179-195, C-Term

Verwendungszweck: Anti-Bid Antibody Picoband®

Sequenz: NQNLFSYVRN LVRNEMD

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Bid Antibody (ABIN3042950). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bid, different from the related rat sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat By Heat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Chou, J. J., Li, H., Salvesen, G. S., Yuan, J., Wagner, G. Solution structure of BID, an intracellular amplifier of apoptotic signaling. Cell 95: 615-624, 1999. 2. Luo, X., Budihardjo, I., Zou, H., Slaughter, C., Wang, X. Bid, a Bcl2 interacting protein, mediates cytochrome c release from mitochondria in response to activation of cell surface death receptors. Cell 94: 481-490, 1998. 3. Wang, K., Yin, X.-M., Copeland, N. G., Gilbert, D. J., Jenkins, N. A., Keck, C. L., Zimonjic, D. B., Popescu, N. C., Korsmeyer, S. J. BID, a proapoptotic BCL-2 family member, is localized to mouse chromosome 6 and human chromosome 22q11. Genomics 53: 235-238, 1998.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-BID Antikörper (ABIN3042950)

Wang, Liu, Mi, Wang, Tang, Zhao, Li: "Sonodynamically induced apoptosis by protoporphyrin IX on hepatoma-22 cells in vitro." in: Ultrasound in medicine & biology, Vol. 36, Issue 4, pp. 667-76, (2010) (PubMed).

Details zu BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Andere Bezeichnung: Bid

Hintergrund:

Synonyms: BH3-interacting domain death agonist,p22 BID,BID,BH3-interacting domain death agonist p15,p15 BID,BH3-interacting domain death agonist p13,p13 BID,BH3-interacting domain death agonist p11,p11 BID,Bid,

Tissue Specificity: Synthesized exclusively by adipocytes and secreted into plasma.

Background: BID (BH3-Interacting Domain Death Agonist), is a pro-apoptotic member of the Bcl-2 protein family. The BCL2 family of proteins consists of both antagonists and agonists that regulate apoptosis and compete through dimerization. By fluorescence in situ hybridization, Wang et al. (1998) mapped the human BID gene to 22q11. Luo et al. (1998) reported the purification of a cytosolic protein that induces cytochrome c release from mitochondria in response to caspase-8, the apical caspase activated by cell surface death receptors such as FAS and TNF.

Sequence Similarities: Contains 1 C1q domain.

Molekulargewicht: 22 kDa

UniProt: P70444

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity