RRM2B Antikörper (C-Term)

Produktnummer ABIN3042928

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch RRM2B in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus.

Kurzübersicht für RRM2B Antikörper (C-Term) (ABIN3042928)

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RRM2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RRM2B Antikörper

Bindungsspezifität: AA 318-332, C-Term

Verwendungszweck: Anti-p53R2/RRM2B Antibody Picoband®

Sequenz: EGKTNFFEKR VSEYQ

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-p53R2/RRM2B Antibody (ABIN3042928). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human p53R2, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Kimura, T., Takeda, S., Sagiya, Y., Gotoh, M., Nakamura, Y., Arakawa, H. Impaired function of p53R2 in Rrm2b-null mice causes severe renal failure through attenuation of dNTP pools. Nature Genet. 34: 440-445, 2003. 2. Kollberg, G., Darin, N., Benan, K., Moslemi, A.-R., Lindal, S., Tulinius, M., Oldfors, A., Holme, E. A novel homozygous RRM2B missense mutation in association with severe mtDNA depletion. Neuromusc. Disord. 19: 147-150, 2009. 3. Takata, A., Kato, M., Nakamura, M., Yoshikawa, T., Kanba, S., Sano, A., Kato, T. Exome sequencing identifies a novel missense variant in RRM2B associated with autosomal recessive progressive external ophthalmoplegia. Genome Biol. 12: R92, 2011.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu RRM2B

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Andere Bezeichnung: RRM2B

Hintergrund:

Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B,1.17.4.1,TP53-inducible ribonucleotide reductase M2 B,p53-inducible ribonucleotide reductase small subunit 2-like protein,p53R2,RRM2B,P53R2,

Tissue Specificity: Widely expressed at a high level in skeletal muscle and at a weak level in thymus. Expressed in epithelial dysplasias and squamous cell carcinoma. .

Background: RRM2B (Ribonucleotide Reductase, M2 B), also known as P53R2, is an enzyme that in humans is encoded by the RRM2B gene. Tanaka et al. (2000) mapped the p53R2 gene to chromosome 8q23.1 by fluorescence in situ hybridization. Tanaka et al. (2000) found that expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wildtype p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and protected cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that had an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair, and cell survival after exposure to various genotoxins.

Sequence Similarities: Belongs to the ribonucleoside diphosphate reductase small chain family.

Molekulargewicht: 83 kDa

Pathways: p53 Signalweg, Negative Regulation of intrinsic apoptotic Signaling