Leupaxin Antikörper (N-Term)

Produktnummer ABIN3042842

Produktdetails anti-Leupaxin Antikörper

Target: Leupaxin (LPXN)

Bindungsspezifität: AA 115-129, N-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leupaxin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Verwendungszweck: Anti-Leupaxin/LPXN Antibody Picoband®

Sequenz: KKHLPDKQDH KASLD

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Leupaxin/LPXN Antibody (ABIN3042842). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Leupaxin, different from the related rat sequence by two amino acids, and from the related mouse sequence by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
1. Lipsky, B. P., Beals, C. R., Staunton, D. E. Leupaxin is a novel LIM domain protein that forms a complex with PYK2. J. Biol. Chem. 273: 11709-11713, 1998. 2. "Entrez Gene: LPXN leupaxin".

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Leupaxin

Target: Leupaxin (LPXN)

Andere Bezeichnung: LPXN (LPXN Produkte)

Hintergrund:

Synonyms: Leupaxin,LPXN,LDLP,

Tissue Specificity: Macrophages, monocytes and osteoclasts (at protein level). Strongly expressed in cells and tissues of hematopoietic origin. Highest expression in lymphoid tissues such as spleen, lymph node, thymus and appendix and in the vascular smooth muscle. Lower levels in bone marrow and fetal liver. Also expressed in peripheral blood lymphocytes and a number of hematopoietic cell lines. Very low levels found in epithelial cell lines. Expressed in prostate cancer (PCa) cells and its expression intensity is ly linked to PCa progression. .

Background: Leupaxin is a protein that in humans is encoded by the LPXN gene. This gene is mapped to 11q12.1. The product encoded by this gene is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity.

Sequence Similarities: Belongs to the paxillin family.

Molekulargewicht: 43 kDa

UniProt: O60711