KIT Antikörper (AA 26-285)

Produktnummer ABIN3042739

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch KIT in WB, FACS, IHC und ICC. Es zeigt Reaktivität gegenüber Proben von Human. Es wurde in 15+ Publikationen zitiert.

Kurzübersicht für KIT Antikörper (AA 26-285) (ABIN3042739)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Produktdetails anti-KIT Antikörper

Bindungsspezifität: AA 26-285

Verwendungszweck: Anti-c-Kit Antibody

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Homologie: Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.

Produktmerkmale: Anti-c-Kit Antibody. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human c-Kit recombinant protein (Position: Q26-S285). Human c-Kit shares 66% amino acid (aa) sequence identity with mouse c-Kit.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mLImmunohistochemistry (Frozen Section), 0.5-1 μg/mL Immunocytochemistry, 0.5-1 μg/mLWestern blot, 0.1-0.5 μg/mLFlow Cytometry (Fixed), 1-3 μg/1x10⁶cells

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-KIT Antikörper (ABIN3042739)

Yang, Zhou, Lan: "Impact of the alterations in the interstitial cells of Cajal on intestinal motility in post-infection irritable bowel syndrome." in: Molecular medicine reports, Vol. 11, Issue 4, pp. 2735-40, (2015) (PubMed).

Li, Wang, Zhao, Yang, Li, Zhao, Yu, Wang, Meng, Liu, Zhu, Gao, Jia, Yang: "Nuclear factor of activated T cells 5 maintained by Hotair suppression of miR-568 upregulates S100 calcium binding protein A4 to promote breast cancer metastasis." in: Breast cancer research : BCR, Vol. 16, Issue 5, pp. 454, (2015) (PubMed).

Xu, Ge, Han, Yang, Su, Zhao, Zhao, Yang, Yang: "High-mobility group box 1 expression and lymph node metastasis in intrahepatic cholangiocarcinoma." in: World journal of gastroenterology : WJG, Vol. 21, Issue 11, pp. 3256-65, (2015) (PubMed).

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." in: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Ban, Fan, Huang, Li, Chen, Zhang, Xu: "Mono-(2-ethylhexyl) phthalate induces injury in human umbilical vein endothelial cells." in: PLoS ONE, Vol. 9, Issue 5, pp. e97607, (2014) (PubMed).

Tang, Li, Yu, Gao, Liu, Chen, Xing, Liu, Yao: "Quercetin prevents ethanol-induced iron overload by regulating hepcidin through the BMP6/SMAD4 signaling pathway." in: The Journal of nutritional biochemistry, Vol. 25, Issue 6, pp. 675-82, (2014) (PubMed).

Cheng, Wu, Gao, Xu: "Analysis of influence of baicalin joint resveratrol retention enema on the TNF-?, SIgA, IL-2, IFN-? of rats with respiratory syncytial virus infection." in: Cell biochemistry and biophysics, Vol. 70, Issue 2, pp. 1305-9, (2014) (PubMed).

Liu, Peng, Lin, Luo, Jiang, Mo, Yung: "Effect of rhynchophylline on the expression of p-CREB and sc-Fos in triatum and hippocampal CA1 area of methamphetamine-induced conditioned place preference rats." in: Fitoterapia, Vol. 92, pp. 16-22, (2014) (PubMed).

Zhang, Li, Xu, Mei, Zhang, Murong, Sun, Tong: "Changes of c-fos, malondialdehyde and lactate in brain tissue after global cerebral ischemia under different brain temperatures." in: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 3, pp. 354-8, (2014) (PubMed).

Liu, Wang, Du, Liu, Liu, Zhang, Zhang, Hou, Liu, Zhang, Yu: "Wnt1 inhibits hydrogen peroxide-induced apoptosis in mouse cardiac stem cells." in: PLoS ONE, Vol. 8, Issue 3, pp. e58883, (2013) (PubMed).

Chuangui, Peng, Zhentao: "The expression of high mobility group box 1 is associated with lymph node metastasis and poor prognosis in esophageal squamous cell carcinoma." in: Pathology oncology research : POR, Vol. 18, Issue 4, pp. 1021-7, (2012) (PubMed).

Bin, Ma, Xu, Shi: "Embryonic hepatocyte transplantation for hepatic cirrhosis: efficacy and mechanism of action." in: World journal of gastroenterology, Vol. 18, Issue 4, pp. 309-22, (2012) (PubMed).

Lan, Yue, Han, Shi, Yang, Pu, Yao, Kang: "Genome-wide identification of TCF7L2/TCF4 target miRNAs reveals a role for miR-21 in Wnt-driven epithelial cancer." in: International journal of oncology, Vol. 40, Issue 2, pp. 519-26, (2011) (PubMed).

Bao, Liu, Qin, Xu, Hei, Jaber, Chen: "An immunohistochemical study of S-100 protein in the intestinal tract of Chinese soft-shelled turtle, Pelodiscus sinensis." in: Research in veterinary science, Vol. 91, Issue 3, pp. e16-24, (2011) (PubMed).

Ge, Zhang, Ye, Tang, Wu: "Ginsenosides promote proliferation of chicken primordial germ cells via PKC-involved activation of NF-kappaB." in: Cell biology international, Vol. 31, Issue 10, pp. 1251-6, (2007) (PubMed).

Details zu KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Andere Bezeichnung: KIT

Hintergrund:

Background: SCFR (Mast/stem cell growth factor receptor), also known as KIT ONCOGENE or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.

Gene Full Name: KIT proto-oncogene, receptor tyrosine kinase

Sequence Similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.

Molekulargewicht: 109 kDa

Gen-ID: 3815

UniProt: P10721

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity