Cullin 1 Antikörper (N-Term)

Produktnummer ABIN3042671

Produktdetails anti-Cullin 1 Antikörper

Target: Cullin 1 (CUL1)

Bindungsspezifität: AA 34-52, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cullin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Cullin-1(Cul1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: RQSMAKSRYM ELYTHVYNY

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Cullin-1(Cul1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: cullin 1
Protein Name: Cullin-1(CUL-1)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Cullin 1(34-52aa RQSMAKSRYMELYTHVYNY), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Cullin 1

Target: Cullin 1 (CUL1)

Andere Bezeichnung: Cul1 (CUL1 Produkte)

Synonyme: LOC100282292 antikoerper, Cullin-1 antikoerper, Cul1 antikoerper, zgc:55747 antikoerper, zgc:77500 antikoerper, wu:fc13a10 antikoerper, cullin-1 antikoerper, ATCUL1 antikoerper, AUXIN RESISTANT 6 antikoerper, AXR6 antikoerper, T10P11.26 antikoerper, T10P11_26 antikoerper, cullin 1 antikoerper, cul1 antikoerper, wu:fi43h07 antikoerper, wu:fj43a08 antikoerper, zgc:111899 antikoerper, zgc:55483 antikoerper, Cul-1 antikoerper, cullin 1 antikoerper, cullin-1 antikoerper, Cullin 1 antikoerper, Cullin-1 antikoerper, cullin 1a antikoerper, cullin 1 S homeolog antikoerper, cullin 1b antikoerper, CUL1 antikoerper, LOC100282292 antikoerper, cul1 antikoerper, ATEG_00491 antikoerper, EDI_072700 antikoerper, EDI_264400 antikoerper, SJAG_02591 antikoerper, BDBG_03785 antikoerper, Cul1 antikoerper, cul1a antikoerper, cul1.S antikoerper, cul-1 antikoerper, cul1b antikoerper

Hintergrund: Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF(SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human cullin 1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1.

Synonyms: CUL 1 antibody|CUL-1 antibody|CUL1 antibody|CUL1_HUMAN antibody|Cullin-1 antibody|Cullin1 antibody|MGC149834 antibody|MGC149835 antibody

UniProt: Q13616

Pathways: Zellzyklus, Hedgehog Signalweg, Mitotic G1-G1/S Phases, Regulation of Cell Size