Cullin 1 Antikörper (N-Term)

Produktnummer ABIN3042671

Der Kaninchen Polyklonal Anti-Cullin 1-Antikörper wurde für WB und IHC validiert. Er ist geeignet, Cullin 1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Cullin 1 Antikörper (N-Term) (ABIN3042671)

Target: Cullin 1 (CUL1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cullin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Cullin 1 Antikörper

Bindungsspezifität: AA 34-52, N-Term

Verwendungszweck: Anti-Cullin 1/Cul1 Antibody Picoband®

Sequenz: RQSMAKSRYM ELYTHVYNY

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Cullin 1/Cul1 Antibody (ABIN3042671). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Cullin 1, identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Kipreos ET, Lander LE, Wing JP, et al. (1996). "cul-1 is required for cell cycle exit in C. elegans and identifies a novel gene family.". Cell 85 (6): 829-39. Bonaldo MF, Lennon G, Soares MB (1997). 2. "Normalization and subtraction: two approaches to facilitate gene discovery.". Genome Res. 6 (9): 791-806. 3. Michel, J. J., Xiong, Y. Human CUL-1, but not other cullin family members, selectively interacts with SKP1 to form a complex with SKP2 and cyclin A. Cell Growth Differ. 9: 435-449, 1998.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu Cullin 1

Target: Cullin 1 (CUL1)

Andere Bezeichnung: Cul1

Hintergrund:

Synonyms: Cullin-1,CUL-1,CUL1,

Tissue Specificity: Expressed in lung fibroblasts. .

Background: Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human cullin 1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1.

Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Molekulargewicht: 90 kDa

UniProt: Q13616

Pathways: Zellzyklus, Hedgehog Signalweg, Mitotic G1-G1/S Phases, Regulation of Cell Size