TBK1 Antikörper (C-Term)

Produktnummer ABIN3042649

Produktdetails anti-TBK1 Antikörper

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Bindungsspezifität: AA 577-590, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TBK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-NAK/TBK1 Antibody Picoband®

Sequenz: YNEEQIHKFD KQKL

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-NAK/TBK1 Antibody (ABIN3042649). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NAK, identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Rat, Mouse
Western blot, 0.1-0.5 μg/mL, Human, Rat, Mouse
1. Barbie, D. A., Tamayo, P., Boehm, J. S., Kim, S. Y., Moody, S. E., Dunn, I. F., Schinzel, A. C., Sandy, P., Meylan, E., Scholl, C., Frohling, S., Chan, E. M., and 23 others. Systematic RNA interference reveals that oncogenic KRAS-driven cancers require TBK1. Nature 462: 108-112, 2009. 2. Chien, Y., Kim, S., Bumeister, R., Loo, Y.-M., Kwon, S. W., Johnson, C. L., Balakireva, M. G., Romeo, Y., Kopelovich, L., Gale, M., Jr., Yeaman, C., Camonis, J. H., Zhao, Y., White, M. A. RalB GTPase-mediated activation of the I-kappa-B family kinase TBK1 couples innate immune signaling to tumor cell survival. Cell 127: 157-170, 2006. 3. Fingert, J. H., Robin, A. L., Stone, J. L., Roos, B. R., Davis, L. K., Scheetz, T. E., Bennett, S. R., Wassink, T. H., Kwon, Y. H., Alward, W. L. M., Mullins, R. F., Sheffield, V. C., Stone, E. M. Copy number variations on chromosome 12q14 in patients with normal tension glaucoma. Hum. Molec. Genet. 20: 2482-2494, 2011. 4. Fujita, F., Taniguchi, Y., Kato, T., Narita, Y., Furuya, A., Ogawa, T., Sakurai, H., Joh, T., Itoh, M., Delhase, M., Karin, M., Nakanishi, M. Identification of NAP1, a regulatory subunit of I-kappa-B kinase-related kinases that potentiates NF-kappa-B signaling. Molec. Cell. Biol. 23: 7780-7793, 2003.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu TBK1

Target: TBK1 (TANK-Binding Kinase 1 (TBK1))

Andere Bezeichnung: TBK1 (TBK1 Produkte)

Hintergrund:

Synonyms: Serine/threonine-protein kinase TBK1,2.7.11.1,NF-kappa-B-activating kinase,T2K,TANK-binding kinase 1,TBK1,NAK,

Tissue Specificity: Ubiquitous with higher expression in testis. Expressed in the ganglion cells, nerve fiber layer and microvasculature of the retina. .

Background: Serine/threonine-protein kinase TBK1, also called TANK-binding kinase 1 or NF-kappa-B-activating kinase is an enzyme that in humans is encoded by the TBK1 gene. The gene was assigned to human chromosome 12q14.2. Serine/threonine kinase plays an essential role in regulating inflammatory responses to foreign agents. TBK1 and NF-kappa-B signaling are essential in KRAS mutant tumors, and established a general approach for the rational identification of codependent pathways in cancer.

Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.

Molekulargewicht: 100 kDa

Pathways: TLR Signalweg, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interaktom