SIRT1 Antikörper (AA 112-311)

Produktnummer ABIN3042483

Dieses Anti-SIRT1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von SIRT1 in WB, IF und ICC. Geeignet für Human. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für SIRT1 Antikörper (AA 112-311) (ABIN3042483)

Target: SIRT1 (Sirtuin 1 (SIRT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-SIRT1 Antikörper

Bindungsspezifität: AA 112-311

Verwendungszweck: Anti-SIRT1 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-SIRT1 Antibody Picoband® (ABIN3042483). Tested in IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human SIRT1 recombinant protein (Position: R112-K311). Human SIRT1 shares 90% amino acid (aa) sequence identity with mouse SIRT1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
1. Wilson BJ, Tremblay AM, Deblois G, Sylvain-Drolet G, Giguère V (Jul 2010). "An acetylation switch modulates the transcriptional activity of estrogen-related receptor alpha.". Mol. Endocrinol. 24 (7): 1349-58. 2. Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P (Mar 2005). "Nutrient control of glucose homeostasis through a complex of PGC-1alpha and SIRT1". Nature 434 (7029): 113-8. 3. Nemoto S, Fergusson MM, Finkel T (Apr 2005). "SIRT1 functionally interacts with the metabolic regulator and transcriptional coactivator PGC-1 alpha". J Biol Chem. 280 (16): 16456-60.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-SIRT1 Antikörper (ABIN3042483)

Hou, Zheng, Li, Gao, Zhang: "The protective effect of glycyrrhizic acid on renal tubular epithelial cell injury induced by high glucose." in: International journal of molecular sciences, Vol. 15, Issue 9, pp. 15026-43, (2015) (PubMed).

Details zu SIRT1

Target: SIRT1 (Sirtuin 1 (SIRT1))

Andere Bezeichnung: SIRT1

Hintergrund:

Synonyms: NAD-dependent protein deacetylase sirtuin-1,hSIRT1,3.5.1.-,Regulatory protein SIR2 homolog 1,SIR2-like protein 1,hSIR2,SirtT1 75 kDa fragment,75SirT1,SIRT1,SIR2L1,

Tissue Specificity: Widely expressed. .

Background: Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene. It is mapped to 10q21.3. Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity. Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.

Sequence Similarities: Belongs to the sirtuin family. Class I subfamily.

Molekulargewicht: 120 kDa

Gen-ID: 23411

Pathways: MAPK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling