Gelsolin Antikörper (AA 580-782)

Produktnummer ABIN3042436

Produktdetails anti-Gelsolin Antikörper

Target: Gelsolin (GSN)

Bindungsspezifität: AA 580-782

Reaktivität: Human, Ratte, Maus, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Gelsolin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Verwendungszweck: Anti-Gelsolin/GSN Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Gelsolin/GSN Antibody Picoband® (ABIN3042436). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Gelsolin recombinant protein (Position: E580-A782). Human Gelsolin shares 94% and 95% amino acid (aa) sequences identity with mouse and rat Gelsolin, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat, Monkey
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Kamada, S., Kusano, H., Fujita, H., Ohtsu, M., Koya, R. C., Kuzumaki, N., Tsujimoto, Y. A cloning method for caspase substrates that uses the yeast two-hybrid system: cloning of the antiapoptotic gene gelsolin. Proc. Nat. Acad. Sci. 95: 8532-8537, 1998. 2. Kim, J., Lee, J. E., Heynen-Genel, S., Suyama, E., Ono, K., Lee, K., Ideker, T., Aza-Blanc, P., Gleeson, J. G. Functional genomic screen for modulators of ciliogenesis and cilium length. Nature 464: 1048-1051, 2010. 3. Vasconcellos, C. A., Allen, P. G., Wohl, M. E., Drazen, J. M., Janmey, P. A., Stossel, T. P. Reduction in viscosity of cystic fibrosis sputum in vitro by gelsolin. Science 263: 969-971, 1994.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Gelsolin Antikörper (ABIN3042436)

Guo, Cui, Jiang, Zhang, Jiang: "Proteomics of acute heart failure in a rat post-myocardial infarction model." in: Molecular medicine reports, Vol. 16, Issue 2, pp. 1946-1956, (2018) (PubMed).

Details zu Gelsolin

Target: Gelsolin (GSN)

Andere Bezeichnung: GSN (GSN Produkte)

Hintergrund:

Synonyms: Gelsolin,AGEL,Actin-depolymerizing factor,ADF,Brevin,GSN,

Tissue Specificity: Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.

Background: Gelsolin, also known as GNS or brevin, is an actin-binding protein that is a key regulator of actin filament assembly and disassembly. Gelsolin is one of the most potent members of the actin-severing gelsolin/villin superfamily. The gene was assigned to human chromosome 9q33.2. It is the principal intracellular and extracellular actin-severing protein. Gelsolin and Gc protein together constitute the extracellular actin-scavenger system which prevents the toxic effects of actin release into the extracellular space under circumstances of cell necrosis. Gelsolin may have therapeutic potential as a mucolytic agent in CF patients. The antiapoptotic activity of gelsolin seems to prevent a step leading to cytochrome c release from the mitochondria into the cytosol.

Sequence Similarities: Belongs to the villin/gelsolin family.

Molekulargewicht: 86 kDa

Gen-ID: 2934

UniProt: P06396

Pathways: Caspase Kaskade in der Apoptose, Regulation of Actin Filament Polymerization, Autophagie