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GCLC Antikörper (AA 437-637)

GCLC Reaktivität: Human, Maus, Ratte WB, IHC, FACS Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN3042420
  • Target Alle GCLC Antikörper anzeigen
    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
    Bindungsspezifität
    • 9
    • 8
    • 7
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 437-637
    Reaktivität
    • 57
    • 19
    • 18
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Wirt
    • 56
    • 3
    Kaninchen
    Klonalität
    • 47
    • 12
    Polyklonal
    Konjugat
    • 33
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Dieser GCLC Antikörper ist unkonjugiert
    Applikation
    • 41
    • 26
    • 19
    • 14
    • 11
    • 7
    • 7
    • 4
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Verwendungszweck
    Anti-GCLC Antibody Picoband®
    Kreuzreaktivität (Details)
    No cross-reactivity with other proteins
    Produktmerkmale
    Anti-GCLC Antibody Picoband® (ABIN3042420). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
    Aufreinigung
    Immunogen affinity purified.
    Immunogen
    E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.
    Isotyp
    IgG
    Top Product
    Discover our top product GCLC Primärantikörper
  • Applikationshinweise
    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
    1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35, 1992. 2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M. L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.
    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    Konzentration
    500 μg/mL
    Buffer
    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
    Handhabung
    Avoid repeated freezing and thawing.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Ni, Wang, Qiu, Pang, Song, Guo: "Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).

    Jin, Li, Zhang, Wu, Feng, Zhang, Shen: "Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway." in: Neural regeneration research, Vol. 11, Issue 7, pp. 1090-8, (2016) (PubMed).

  • Target
    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
    Andere Bezeichnung
    GCLC (GCLC Produkte)
    Hintergrund

    Synonyms: Glutamate--cysteine ligase catalytic subunit,6.3.2.2,GCS heavy chain,Gamma-ECS,Gamma-glutamylcysteine synthetase,GCLC,GLCL, GLCLC,

    Tissue Specificity: T-cells and endothelial cells.

    Background: GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

    Sequence Similarities: Belongs to the glutamate--cysteine ligase type 3 family.

    Molekulargewicht
    72 kDa
    Gen-ID
    2729
    UniProt
    P48506
    Pathways
    Cell RedoxHomeostasis
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