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GCLC Antikörper (AA 437-637)

Dieses Anti-GCLC-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GCLC in WB, IHC und FACS. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 2+ Publikationen zitiert.
Produktnummer ABIN3042420

Kurzübersicht für GCLC Antikörper (AA 437-637) (ABIN3042420)

Target

Alle GCLC Antikörper anzeigen
GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Reaktivität

  • 62
  • 25
  • 23
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Maus, Ratte

Wirt

  • 67
  • 5
Kaninchen

Klonalität

  • 57
  • 15
Polyklonal

Konjugat

  • 46
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
Dieser GCLC Antikörper ist unkonjugiert

Applikation

  • 54
  • 30
  • 28
  • 16
  • 15
  • 15
  • 11
  • 8
  • 2
  • 1
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 9
    • 8
    • 7
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 437-637

    Verwendungszweck

    Anti-GCLC Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-GCLC Antibody Picoband® (ABIN3042420). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human GCLC recombinant protein (Position: E437-N637). Human GCLC shares 94% amino acid (aa) sequence identity with both mouse and rat GCLC.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
    1. Gipp, J. J., Chang, C., Mulcahy, R. T.Cloning and nucleotide sequence of a full-length cDNA for human liver gamma-glutamylcysteine synthetase.Biochem. Biophys. Res. Commun. 185: 29-35, 1992. 2. Sierra-Rivera, E., Summar, M. L., Dasouki, M., Krishnamani, M. R. S., Phillips, J. A., Freeman, M. L.Assignment of the gene (GLCLC) that encodes the heavy subunit of gamma-glutamylcysteine synthetase to human chromosome 6.Cytogenet. Cell Genet. 70: 278-279, 1995.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Ni, Wang, Qiu, Pang, Song, Guo: "Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).

    Jin, Li, Zhang, Wu, Feng, Zhang, Shen: "Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway." in: Neural regeneration research, Vol. 11, Issue 7, pp. 1090-8, (2016) (PubMed).

  • Target

    GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

    Andere Bezeichnung

    GCLC

    Hintergrund

    Synonyms: Glutamate--cysteine ligase catalytic subunit,6.3.2.2,GCS heavy chain,Gamma-ECS,Gamma-glutamylcysteine synthetase,GCLC,GLCL, GLCLC,

    Tissue Specificity: T-cells and endothelial cells.

    Background: GCLC, also named Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.

    Sequence Similarities: Belongs to the glutamate--cysteine ligase type 3 family.

    Molekulargewicht

    72 kDa

    Gen-ID

    2729

    UniProt

    P48506

    Pathways

    Cell RedoxHomeostasis
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