GLI3 Antikörper (AA 41-57)
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- Target Alle GLI3 Antikörper anzeigen
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
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Bindungsspezifität
- AA 41-57
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Reaktivität
- Human, Ratte, Hund, Huhn, Affe, Wachtel, Frosch
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser GLI3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Spezifität
- This antibody recognises GLI-Kruppel Family Member GLI3.
- Kreuzreaktivität (Details)
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Species reactivity (expected):Chimpanzee, Chicken, Canine (Dog), Frog, Monkey, Quail and Rat.
Species reactivity (tested):Human. - Aufreinigung
- Immunoaffinity Chromatography
- Immunogen
- Synthetic Peptide -KLH conjugated corresponding to Amino acids 41-57 of Human Gli-3 protein.
- Top Product
- Discover our top product GLI3 Primärantikörper
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- Applikationshinweise
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ELISA (1/6000-1/30000). Western Blot (1/500-1/2000). Immunohistochemistry on Paraffin Sections (1.25 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1,0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid cycles of freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.
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- Target
- GLI3 (GLI Family Zinc Finger 3 (GLI3))
- Andere Bezeichnung
- GLI3 (GLI3 Produkte)
- Synonyme
- ACLS antikoerper, GCPS antikoerper, GLI3-190 antikoerper, GLI3FL antikoerper, PAP-A antikoerper, PAPA antikoerper, PAPA1 antikoerper, PAPB antikoerper, PHS antikoerper, PPDIV antikoerper, xgli3 antikoerper, AI854843 antikoerper, AU023367 antikoerper, Bph antikoerper, Pdn antikoerper, Xt antikoerper, add antikoerper, im:7142603 antikoerper, GLI3 antikoerper, GLI family zinc finger 3 antikoerper, GLI family zinc finger 3 L homeolog antikoerper, GLI-Kruppel family member GLI3 antikoerper, GLI3 antikoerper, Gli3 antikoerper, gli3.L antikoerper, gli3 antikoerper
- Hintergrund
- Gli-3 (also known as Zinc Finger Protein Gli-3 or GLI-Kruppel family member GLI-3) belongs to the GLI C2H2-type zinc-finger protein family and contains 5 C2H2-type zinc fingers. Gli-3 is very important for normal limb and brain development and is implicated in the transduction of Shh signal. Gli-3 is a nuclear protein expressed in a wide variety of normal adult tissues, including lung, colon, spleen, placenta, testis, and myometrium. Defects in Gli-3 are the cause of Greig cephalo-poly-syndactyly syndrome (GCPS), an autosomal dominant disorder-affecting limb and cranio-facial development. Two isoforms of human Gli-3 have been reported. One is the full-length protein at ~170-190 kDa and the other is a truncated isoform at ~80 kDa.Synonyms: GLI-3, Zinc finger protein GLI3
- Gen-ID
- 2737
- NCBI Accession
- NP_000159
- UniProt
- P10071
- Pathways
- Hedgehog Signalweg
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