14-3-3 sigma/SFN Antikörper (AA 140-156)

Produktnummer ABIN3032756

Der Kaninchen Polyklonal Anti-14-3-3 sigma/SFN-Antikörper wurde für WB, IHC (p) und ICC validiert. Er ist geeignet, 14-3-3 sigma/SFN in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für 14-3-3 sigma/SFN Antikörper (AA 140-156) (ABIN3032756)

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser 14-3-3 sigma/SFN Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Produktdetails anti-14-3-3 sigma/SFN Antikörper

Bindungsspezifität: AA 140-156

Aufreinigung: Antigen affinity

Immunogen: Amino acids 140-156 (KKRIIDSARSAYQEAMD) were used as the immunogen for this 14-3-3 sigma antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the 14-3-3 sigma antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the 14-3-3 sigma antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu 14-3-3 sigma/SFN

Target: 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))

Andere Bezeichnung: 14-3-3 sigma

Hintergrund: Stratifin (SFN), also known as 14-3-3 sigma, is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8 kb upstream of its transcription start site. Leffers et al.(1993) obtained a peptide sequence and subsequently cloned a T-cell cDNA of the 14-3-3 family of conserved proteins. The protein called stratifin was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells. It was most abundant in tissues enriched in stratified keratinizing epithelium.

UniProt: P31947

Pathways: p53 Signalweg, Myometrial Relaxation and Contraction