RRM2B Antikörper (C-Term)

Produktnummer ABIN3032475

Der Kaninchen Polyklonal anti-RRM2B Antikörper (ABIN3032475) detektiert spezifisch RRM2B in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Maus.

Kurzübersicht für RRM2B Antikörper (C-Term) (ABIN3032475)

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RRM2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RRM2B Antikörper

Bindungsspezifität: C-Term

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the C-terminus of human p53 reductase small subunit 2 (EGKTNFFEKRVSEYQ) was used as the immunogen for this p53R2 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the p53R2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the p53R2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu RRM2B

Target: RRM2B (Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B))

Andere Bezeichnung: p53R2

Hintergrund: Ribonucleotide Reductase, M2 B, also known as P53R2, is an enzyme that in humans is encoded by the RRM2B gene. Tanaka et al.(2000) mapped the gene to chromosome 8q23.1 by fluorescence in situ hybridization. Tanaka et al.(2000) found that expression of p53R2, but not R2, was induced by ultraviolet and gamma-irradiation and adriamycin treatment in a wildtype p53-dependent manner. Induction of p53R2 in p53-deficient cells caused G2/M arrest and protected cells from death in response to adriamycin. Inhibition of endogenous p53R2 expression in cells that had an intact p53-dependent DNA damage checkpoint reduced ribonucleotide reductase activity, DNA repair, and cell survival after exposure to various genotoxins.

UniProt: Q7LG56

Pathways: p53 Signalweg, Negative Regulation of intrinsic apoptotic Signaling