MCL-1 Antikörper (AA 303-325)

Produktnummer ABIN3031296

Der Kaninchen Polyklonal anti-MCL-1 Antikörper (ABIN3031296) detektiert spezifisch MCL-1 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für MCL-1 Antikörper (AA 303-325) (ABIN3031296)

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MCL-1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-MCL-1 Antikörper

Bindungsspezifität: AA 303-325

Verwendungszweck: MCL-1 Antibody

Aufreinigung: Antigen affinity

Immunogen: Amino acids 303-325 (RDWLVKQRGWDGFVEFFHVEDLE-human) were used as the immunogen for this MCL-1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the MCL-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: After reconstitution, the MCL-1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Details zu MCL-1

Target: MCL-1 (MCL1) (Induced Myeloid Leukemia Cell Differentiation Protein Mcl-1 (MCL1))

Andere Bezeichnung: MCL-1

Hintergrund: BCL2L3, also known as Myeloid cell leukemia sequence 1, encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products(isoform 2 and isoform 3) promote apoptosis and are death-inducing. Using the methods of somatic cell hybrid analysis and fluorescence in situ hybridization), the MCL-1 gene is mapped to human 1q21. MCL-1is a critical and specific regulator essential for ensuring the homeostasis of early hematopoietic progenitors. Phosphorylation of MCL-1 directs its interaction with the tumor suppressor protein FBW7, which is the substrate-binding component of a ubiquitin ligase complex. The polyubiquitylation of MCL-1 then targets it for proteasomal degradation. Degradation was blocked in patient-derived tumor cells that lacked FBW7 or had loss-of-function mutations in FBW7, conferring resistance to antitubulin agents and promoting chemotherapeutic-induced polyploidy.

UniProt: Q07820

Pathways: MAPK Signalweg