BMI1 Antikörper

Produktnummer ABIN303071

Der Ziege Polyklonal anti-BMI1 Antikörper wird verwendet zum Nachweis von BMI1 in Proben von Human, Maus, Ratte, Hund, Rind (Kuh), Huhn, Affe, Cat und Frosch. Er wurde validiert für WB, IF, IHC (p) und EIA.

Kurzübersicht für BMI1 Antikörper (ABIN303071)

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Reaktivität: Human, Maus, Ratte, Hund, Rind (Kuh), Huhn, Affe, Cat, Frosch

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser BMI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-BMI1 Antikörper

Spezifität: This antibody reacts to DNA-binding protein Bmi-1.

Aufreinigung: Immunoaffinity Chromatography

Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on paraffin sections: 2,5 μg/mL. Western Blot: 1: 500 - 1: 3000. ELISA: 1: 5000 - 30000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.9 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Details zu BMI1

Target: BMI1 (BMI1 Polycomb Ring Finger Oncogene (BMI1))

Andere Bezeichnung: BMI-1 / RNF51

Hintergrund: The Bmi-1 oncogene (also known as polycomb group ring finger 4, MGC12685, murine leukemia viral (bmi 1) oncogene homolog, oncogene BMI 1, polycomb complex protein BMI 1 and RNF51) induces telomerase activity and immortalizes human mammary epithelial cells. Bmi-1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway. The polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi-1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation.Synonyms: BMI1, PCGF4, Polycomb group RING finger protein 4, RING finger protein 51

Gen-ID: 100532731

NCBI Accession: NP_001190991

UniProt: P35226

Pathways: Zellzyklus, Autophagie