Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN3027014

Dieser Maus Monoklonal Antikörper detektiert spezifisch Topoisomerase II alpha in WB, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN3027014)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: TPM2A-2

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Verwendungszweck: Topoisomerase II alpha Antibody / TOP2A (azide and preservative free)

Produktmerkmale: This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this Topoisomerase II alpha antibody.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Topoisomerase II alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: Topoisomerase II alpha

Hintergrund: This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Pathways: Zellzyklus, Mitotic G1-G1/S Phases