Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN3027012

Der Maus Monoklonal anti-Topoisomerase II alpha Antikörper wird verwendet zum Nachweis von Topoisomerase II alpha in Proben von Human. Er wurde validiert für WB, IHC und IF.

Kurzübersicht für Topoisomerase II alpha Antikörper (AA 1352-1493) (ABIN3027012)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Carrier-free

Klon: TPM2A-1

Produktdetails anti-Topoisomerase II alpha Antikörper

Bindungsspezifität: AA 1352-1493

Verwendungszweck: TOP2A Antibody / Topoisomerase II alpha (azide and preservative free)

Produktmerkmale: This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant partial protein (amino acids 1352-1493) was used as the immunogen for this TOP2A antibody.

Isotyp: IgG2b, kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TOP2A antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue requires boiling sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the TOP2A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A

Hintergrund: This mAb recognizes a 170 kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

UniProt: P11388

Pathways: Zellzyklus, Mitotic G1-G1/S Phases