Cyclin B1 Antikörper

Produktnummer ABIN3026645

Dieser Maus Monoklonal Antikörper detektiert spezifisch Cyclin B1 in IF, FACS und IP. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Hamster.

Kurzübersicht für Cyclin B1 Antikörper (ABIN3026645)

Target: Cyclin B1 (CCNB1)

Reaktivität: Human, Maus, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin B1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunoprecipitation (IP)

Klon: V92-1

Produktdetails anti-Cyclin B1 Antikörper

Produktmerkmale: This antibody recognizes a protein of 55-62 kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.

Aufreinigung: Protein G affinity chromatography

Immunogen: Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IP: 1-2 μg/500 μg protein--precipitates active Cyclin B1 complexes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cyclin B1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Cyclin B1

Target: Cyclin B1 (CCNB1)

Andere Bezeichnung: Cyclin B1

Hintergrund: This antibody recognizes a protein of 55-62  kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.

Gen-ID: 891

Pathways: Zellzyklus, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase