Cyclin D1 Antikörper (full length)

Produktnummer ABIN3026636

Dieser Maus Monoklonal Antikörper detektiert spezifisch Cyclin D1 in WB, FACS, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für Cyclin D1 Antikörper (full length) (ABIN3026636)

Target: Cyclin D1 (CCND1)

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cyclin D1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Güteklasse: Carrier-free

Klon: DCS-6

Produktdetails anti-Cyclin D1 Antikörper

Bindungsspezifität: full length

Verwendungszweck: Cyclin D1 Antibody (azide and preservative free)

Produktmerkmale: This antibody recognizes a protein of 36 kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Aufreinigung: Protein G affinity chromatography

Immunogen: Human recombinant full length protein was used as the immunogen for this Cyclin D1 antibody.

Isotyp: IgG2a, kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Cyclin D1 antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Cyclin D1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Cyclin D1

Target: Cyclin D1 (CCND1)

Andere Bezeichnung: Cyclin D1

Hintergrund: This antibody recognizes a protein of 36 kDa, identified as Cyclin D1. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms and a key cell cycle regulator. This antibody neutralizes the activity of Cyclin D1 in vivo. About 60 % of mantle cell lymphomas (MCL) contain a t(11, 14)(q13, q32) translocation resulting in over-expression. Cyclin D1 antibody is useful in identifying mantle cell lymphomas, which stain positive, from CLL/SLL and follicular lymphomas, which stain negative. Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, ER-Nucleus Signaling