FOXA1 Antikörper (AA 372-472)

Produktnummer ABIN3026289

Der Maus Monoklonal Anti-FOXA1-Antikörper wurde für FACS, IF und IHC (p) validiert. Er ist geeignet, FOXA1 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für FOXA1 Antikörper (AA 372-472) (ABIN3026289)

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser FOXA1 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: FHBA1-1

Produktdetails anti-FOXA1 Antikörper

Bindungsspezifität: AA 372-472

Aufreinigung: Protein G affinity chromatography

Immunogen: A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the FOXA1 antibody to be titered up or down for optimal performance.

1. Staining of FFPE tissue is enhanced by boiling sections in 10  mM Tris with 1  mM EDTA,  pH 9 for 10-20 min followed by cooling at RT for 20 min.\. FACS: 0.5-1 μg/10^6 cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu FOXA1

Target: FOXA1 (Forkhead Box A1 (FOXA1))

Andere Bezeichnung: FOXA1

Hintergrund: The transcription factor Forkhead-box A1, also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors. HNF-1 (alpha and beta), HNF-3 (alpha, beta and gamma), HNF-4 (alpha and gamma), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. Its expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Gen-ID: 3169

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis