TGFA Antikörper (AA 34-43)

Produktnummer ABIN3026164

Dieses Anti-TGFA-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von TGFA in IF, FACS und IHC (p). Geeignet für Human, Kaninchen und Zebrafisch (Danio rerio).

Kurzübersicht für TGFA Antikörper (AA 34-43) (ABIN3026164)

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Reaktivität: Human, Kaninchen, Zebrafisch (Danio rerio)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFA Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 1E8-G6

Produktdetails anti-TGFA Antikörper

Bindungsspezifität: AA 34-43

Aufreinigung: PEG precipitation

Immunogen: A 10-amino acid synthetic peptide (aa 34-43) from human TGF alpha was used as the immunogen for this antibody.

Isotyp: IgM kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (Formalin-paraffin): 1-2 μg/mL for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the TGF alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Andere Bezeichnung: TGF alpha

Hintergrund: TGF alpha is a growth factor with 33 % homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. This antibody reacts with the C-terminus of TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. Staining with the antibody is completely blocked by the peptide used for raising this antibody.

Gen-ID: 7039

Pathways: NF-kappaB Signalweg, RTK Signalweg, EGFR Signaling Pathway