Prostate Specific Antigen Antikörper
Kurzübersicht für Prostate Specific Antigen Antikörper (ABIN3026030)
Target
Alle Prostate Specific Antigen (PSA) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Aufreinigung
- Protein G affinity chromatography
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Immunogen
- PSA (p30) from human sperm plasma was used as the immunogen for the Prostate Specific Antigen antibody cocktail.
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Isotyp
- IgG
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Applikationshinweise
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Optimal dilution of the Prostate Specific Antigen antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) -
Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Konzentration
- 1 mg/mL
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Buffer
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
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Konservierungsmittel
- Azide free
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Lagerung
- 4 °C,-20 °C
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Informationen zur Lagerung
- Store the Prostate Specific Antigen antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Prostate Specific Antigen (PSA)
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Andere Bezeichnung
- Prostate Specific Antigen
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Hintergrund
- Recognizes a single protein of 33-34 kDa, identified as the prostate specific antigen (PSA). This mAb is highly specific to PSA and stains prostatic secretory and ductal epithelium in both normal and neoplastic tissues. PSA is a chymotrypsin-like serine protease (kallikrein family) exclusively produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia.
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Pathways
- Komplementsystem
Target
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